软组织肉瘤的流式细胞术S期分数：对160例患者的预后重要性进行分析
Flow cytometric S-phase fraction in soft-tissue sarcoma: prognostic importance analysed in 160 patients.
作者信息
Gustafson P, Fernö M, Akerman M, Baldetorp B, Willén H, Killander D, Rydholm A
机构信息
Department of Orthopedics, University Hospital, Lund, Sweden.
出版信息
Br J Cancer. 1997;75(1):94-100. doi: 10.1038/bjc.1997.15.
We could determine the S-phase fraction (SPF) by flow cytometric DNA analysis of paraffin archival material in 160 of 260 patients with soft-tissue sarcoma of extremity and trunk wall. The prognostic value of SPF was compared with other clinicopathological factors. The median follow-up time was 16 (6-31) years. In a univariate analysis, deep tumour location, increasing tumour size and histological malignancy grade, microscopic tumour necrosis, vascular invasion, DNA non-diploidy and high SPF (>3.0%) were associated with poor metastasis-free survival. In a multivariate analysis, microscopic tumour necrosis and high SPF were independently prognostic for metastasis. Used in combination with tumour size, microscopic tumour necrosis and vascular invasion, SPF could identify a group of patients with a 5-year metastasis-free survival rate of 0.97. This group constituted one-quarter of all patients. Patients with low SPF who did recur had a prolonged clinical course both as regards metastases and local recurrence. We conclude that SPF is a valuable adjunct in prognostication in soft-tissue sarcoma.
我们通过流式细胞术DNA分析，对260例四肢和躯干壁软组织肉瘤患者中的160例石蜡存档材料进行分析，从而确定S期分数（SPF）。将SPF的预后价值与其他临床病理因素进行比较。中位随访时间为16（6 - 31）年。单因素分析中，肿瘤位置深、肿瘤大小增加、组织学恶性程度分级、显微镜下肿瘤坏死、血管侵犯、DNA非二倍体和高SPF（>3.0%）与无转移生存期差相关。多因素分析中，显微镜下肿瘤坏死和高SPF是转移的独立预后因素。与肿瘤大小、显微镜下肿瘤坏死和血管侵犯联合使用时，SPF可识别出一组5年无转移生存率为0.97的患者。该组患者占所有患者的四分之一。SPF低但复发的患者在转移和局部复发方面临床病程延长。我们得出结论，SPF是软组织肉瘤预后评估中有价值的辅助指标。
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