Hedley D W
Ludwig Institute for Cancer Research (Sydney Branch), University of Sydney, N.S.W., Australia.
Cytometry. 1989 May;10(3):229-41. doi: 10.1002/cyto.990100302.
The use of paraffin-embedded tissue for flow cytometry is reviewed. A number of technical modifications of the original 1983 method have been described, aimed at improving the accuracy of DNA measurements by minimizing cell debris or reducing coefficients of variation, and at simplifying sample preparation. Over 100 clinical studies have now been reported, mainly assessing the effect of DNA index on prognosis, and those published up until mid-1988 are summarized in an appendix. More recently there have been developments in the use of monoclonal antibodies to measure oncogene products or proliferation markers in addition to DNA content. Detailed clinical evaluation and standardization of these more sophisticated methods is still some way ahead, but as was the case with DNA index, the use of archival material from patients whose outcome is already known should speed this process.
本文综述了石蜡包埋组织在流式细胞术中的应用。针对1983年的原始方法，已描述了一些技术改进，旨在通过减少细胞碎片或降低变异系数来提高DNA测量的准确性，并简化样品制备。目前已报道了100多项临床研究，主要评估DNA指数对预后的影响，截至1988年年中的相关研究已总结在附录中。最近，除了DNA含量外，在使用单克隆抗体测量癌基因产物或增殖标志物方面也有进展。这些更复杂方法的详细临床评估和标准化仍有待时日，但与DNA指数的情况一样，使用已知预后患者的存档材料应能加快这一进程。
