从成年和新生大鼠肝细胞系中分离和鉴定二倍体克隆。
Isolation and characterization of diploid clones from adult and newborn rat liver cell lines.
作者信息
Tokiwa T, Nakabayashi H, Miyazaki M, Sato J
出版信息
In Vitro. 1979 Jun;15(6):393-400. doi: 10.1007/BF02618406.
PMID:90011
Abstract
A high frequency of diploid and near-diploid clones were developed from cell lines derived from adult and newborn rat liver using micropipettes. There were some differences in morphology, biochemical properties and growth rate between clones. Cloned cells had low levels of tyrosine transaminase activity, glucose-6-phosphatase activity and albumin content. A diploid clone and pseudodiploid clone derived from adult rat liver cell line were positive for alpha-fetoprotein.
摘要
使用微量移液器从成年和新生大鼠肝脏来源的细胞系中培养出了高频率的二倍体和近二倍体克隆。各克隆之间在形态、生化特性和生长速率方面存在一些差异。克隆细胞的酪氨酸转氨酶活性、葡萄糖-6-磷酸酶活性和白蛋白含量水平较低。从成年大鼠肝细胞系衍生的一个二倍体克隆和假二倍体克隆甲胎蛋白呈阳性。
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