Optical demonstration of Chlamydiae in cell cultures by means of fluorochrome 33,258 H.

Fluorochrome 33,258 H, a bisbenzimidazole, was employed for demonstrating Chlamydiae in cell cultures, and proved to be particularly suitable for illustrating the unique intracellular reproduction processes of these organisms. The staining procedure is simple and permits a selective differentiation of chlamydial inclusions and their developmental processes in the cell. Contrary to other staining methods, all stages of the chlamydial reproduction cycle can be illustrated thus permitting the early demonstration of Chlamydiae in the cell. The procedure is suitable not only in experimental investigations on Chlamydiae but also in clinical diagnostics.