缺氧缺血性损伤后的1,6-二磷酸果糖对新生大鼠脑具有保护作用。

Fructose-1,6-bisphosphate after hypoxic ischemic injury is protective to the neonatal rat brain.

作者信息

Sola A, Berrios M, Sheldon R A, Ferriero D M, Gregory G A

机构信息

Department of Pediatrics (Neonatology), University of California, San Francisco 94143-0734, USA.

出版信息

Brain Res. 1996 Nov 25;741(1-2):294-9. doi: 10.1016/s0006-8993(96)00984-5.

DOI:10.1016/s0006-8993(96)00984-5

PMID:9001735

Abstract

Fructose-1,6-bisphosphate (FBP) has been shown to attenuate central nervous system injury in adult animals. We evaluated whether FBP given after an ischemic-hypoxic insult is protective to the developing brain in a neonatal rat model of hypoxia-ischemia. Postnatal day 7 rat pups were subjected to focal ischemia followed by global hypoxia and then administered either FBP or saline intraperitoneally. A dose of 500 mg/kg or greater of FBP significantly reduced the amount of injury such that 55% of FBP- vs. 17% of saline-treated rats had no injury; 6% of FBP- and 47% of saline-treated rats had severe damage (P = 0.004). There was less infarcted brain in FBP-treated rats (12 +/- 11% vs. 37 +/- 32%; P = 0.005); and fewer FBP-treated rats had > 30% ipsilateral cortical injury (12% of FBP- vs. 50% of saline-treated rats; P = 0.002). FBP lowered serum calcium levels during the first 24 h after the insult without significant changes in ionized calcium or osmolarity. These results indicate that FBP treatment administered systemically after hypoxia-ischemia reduces CNS injury in neonatal rats.

摘要

1,6 - 二磷酸果糖（FBP）已被证明可减轻成年动物的中枢神经系统损伤。我们评估了在缺氧缺血性损伤后给予FBP对新生大鼠缺氧缺血性发育中脑是否具有保护作用。出生后第7天的幼鼠先经历局灶性缺血，随后进行全身性缺氧，然后腹腔注射FBP或生理盐水。500mg/kg及以上剂量的FBP显著减少了损伤量，使得接受FBP治疗的大鼠中有55%未出现损伤，而接受生理盐水治疗的大鼠中这一比例为17%；接受FBP治疗的大鼠中有6%出现严重损伤，接受生理盐水治疗的大鼠中这一比例为47%（P = 0.004）。FBP治疗的大鼠脑梗死面积较小（12±11%对37±32%；P = 0.005）；且接受FBP治疗的大鼠中同侧皮质损伤>30%的比例较低（接受FBP治疗的大鼠为12%，接受生理盐水治疗的大鼠为50%；P = 0.002）。FBP在损伤后的最初24小时内降低了血清钙水平，而离子钙或渗透压无显著变化。这些结果表明，缺氧缺血后全身给予FBP治疗可减少新生大鼠的中枢神经系统损伤。