Wu Y, Qui X, Du S, Erickson L
Department of Crop Science, University of Guelph, Ontario, Canada.
Plant Mol Biol. 1996 Dec;32(6):1205-7. doi: 10.1007/BF00041407.
Genomic and cDNA clones have been isolated for a new gene (PO22) expressed in mature pollen of alfalfa. PO22 represents a single- or low-copy gene containing 4 introns, 2 transcriptional start sites, and coding for a predicted protein of 340 amino acids with no signal peptide and a coiled coil structure of 39 amino acids near the carboxyl end. The amino acid sequence of PO22 is 38% identical to that of an early nodule-specific gene (ENOD8), also from alfalfa.
已分离出在苜蓿成熟花粉中表达的一个新基因（PO22）的基因组克隆和cDNA克隆。PO22代表一个单拷贝或低拷贝基因，含有4个内含子、2个转录起始位点，编码一个预测的由340个氨基酸组成的蛋白质，该蛋白质没有信号肽，且在羧基末端附近有一个由39个氨基酸组成的卷曲螺旋结构。PO22的氨基酸序列与同样来自苜蓿的一个早期根瘤特异性基因（ENOD8）的氨基酸序列有38%的同一性。
