Granadino B, Penalva L O, Sánchez L
Centro de Investigaciones Biológicas, Madrid, Spain.
Mol Gen Genet. 1996 Nov 27;253(1-2):26-31. doi: 10.1007/s004380050292.
In Drosophila melanogaster, regulation of the sex determination genes throughout development occurs by sex-specific splicing of their products. The first gene is Sex-lethal(Sxl). The downstream target of Sxl is the gene transformer (tra): the Sxl protein controls the female-specific splicing of the Tra pre-mRNA. The downstream target of the gene tra is the gene double-sex (dsx): the Tra protein of females, controls the female-specific splicing of the Dsx pre-mRNA. We have identified a gene, female-lethal-2-d fl(2) d, whose function is required for the female-specific splicing of Sxl pre-mRNA. In this report we analyze whether the gene fl(2)d is also required for the sex-specific splicing of both Tra and Dsx pre-mRNAs. We found that the Sxl protein is not sufficient for the female-specific splicing of Tra pre-mRNA, the fl(2)d function also being necessary. This gene, however, is not required for the female-specific splicing of Dsx pre-mRNA.
在黑腹果蝇中，整个发育过程中性别决定基因的调控是通过其产物的性别特异性剪接来实现的。第一个基因是性致死基因（Sex-lethal，Sxl）。Sxl的下游靶标是基因transformer（tra）：Sxl蛋白控制Tra前体mRNA的雌性特异性剪接。基因tra的下游靶标是基因双性基因（double-sex，dsx）：雌性的Tra蛋白控制Dsx前体mRNA的雌性特异性剪接。我们鉴定出了一个基因，雌性致死-2-d（female-lethal-2-d，fl(2)d），其功能是Sxl前体mRNA雌性特异性剪接所必需的。在本报告中，我们分析了fl(2)d基因对于Tra和Dsx前体mRNA的性别特异性剪接是否也是必需的。我们发现，Sxl蛋白不足以实现Tra前体mRNA的雌性特异性剪接，fl(2)d的功能也是必需的。然而，该基因对于Dsx前体mRNA的雌性特异性剪接并非必需。
