Brown J, Entrican G
Moredun Research Institute, Edinburgh, UK.
J Comp Pathol. 1996 Nov;115(4):373-83. doi: 10.1016/s0021-9975(96)80072-7.
The long-term anti-chlamydial effects of recombinant ovine interferon gamma (rOvIFN-gamma) were studied in ovine ST-6 fibroblasts infected with the S26/3 strain of Chlamydia psittaci. Chlamydial multiplication was assessed by enzyme-linked immunosorbent assay analysis of supernate lipopolysaccharide, titration of inclusion-forming units in culture supernates, and enumeration of inclusion bodies in cultured cells at 7-day intervals. Concentrations of 250 and 1000 U/ml of rOvIFN-gamma resulted in a microbistatic inhibition of C. psittaci growth, which appeared to become microbicidal when rOvIFN-gamma was maintained in the cultures for 14 days or more. There were no signs of C. psittaci multiplication when cultures were maintained in 25 or 100 U/ml of rOvIFN-gamma. However, subsequent removal of rOvIFN-gamma from these cultures resulted in a re-emergence of viable, infectious chlamydiae, which eventually killed all the fibroblasts. This re-emergence was more rapid in cultures initially treated with 25 U/ml of rOvIFN-gamma than in those treated with 100 U/ml.
在感染鹦鹉热衣原体S26/3菌株的绵羊ST-6成纤维细胞中研究了重组羊γ干扰素（rOvIFN-γ）的长期抗衣原体作用。通过对上清液脂多糖进行酶联免疫吸附测定分析、滴定培养上清液中的包涵体形成单位以及每隔7天对培养细胞中的包涵体进行计数来评估衣原体增殖情况。250和1000 U/ml的rOvIFN-γ浓度导致对鹦鹉热衣原体生长的抑菌作用，当rOvIFN-γ在培养物中维持14天或更长时间时，这种作用似乎会变成杀菌作用。当培养物在25或100 U/ml的rOvIFN-γ中维持时，没有鹦鹉热衣原体增殖的迹象。然而，随后从这些培养物中去除rOvIFN-γ会导致有活力的、具有传染性的衣原体重新出现，最终杀死所有成纤维细胞。在最初用25 U/ml的rOvIFN-γ处理的培养物中，这种重新出现比用100 U/ml处理的培养物更快。
