Graham S P, Jones G E, MacLean M, Livingstone M, Entrican G
Moredun Research Institute, Edinburgh, UK.
J Comp Pathol. 1995 Feb;112(2):185-95. doi: 10.1016/s0021-9975(05)80060-x.
The local production of interferon-gamma (IFN-gamma) in sheep in response to Chlamydia psittaci was measured by cannulation of the efferent lymph duct draining the site of challenge inoculation. Peak production of IFN-gamma (256 U/ml) was detected 24 h after challenge. Based on these physiological data, functional studies were carried out in vitro to determine the effect of recombinant ovine (rOv) IFN-gamma on the multiplication of C. psittaci in ovine fibroblasts. IFN-gamma inhibited the multiplication of C. psittaci in ovine cells over a range of concentrations (250 U/ml to 2.5 U/ml) in a dose-dependent manner. The inhibition of chlamydial multiplication was most pronounced when cells were treated with rOvIFN-gamma for 24 h before infection. The addition of exogenous L-tryptophan (500 micrograms/ml) to cultures within 48th of infection abrogated the anti-chlamydial effect of rOvIFN-gamma thus suggesting that tryptophan deprivation is an anti-chlamydial mechanism induced by rOvIFN-gamma in these ovine cells.
通过对接种攻击部位的输出淋巴管进行插管，测定绵羊对鹦鹉热衣原体产生的干扰素-γ（IFN-γ）的局部产量。攻击后24小时检测到IFN-γ的峰值产量（256 U/ml）。基于这些生理学数据，进行了体外功能研究，以确定重组绵羊（rOv）IFN-γ对鹦鹉热衣原体在绵羊成纤维细胞中增殖的影响。IFN-γ在一系列浓度（250 U/ml至2.5 U/ml）范围内以剂量依赖性方式抑制鹦鹉热衣原体在绵羊细胞中的增殖。当细胞在感染前用rOvIFN-γ处理24小时时，衣原体增殖的抑制最为明显。在感染后48小时内，向培养物中添加外源性L-色氨酸（500微克/毫升）消除了rOvIFN-γ的抗衣原体作用，因此表明色氨酸剥夺是rOvIFN-γ在这些绵羊细胞中诱导的一种抗衣原体机制。
