Owunwanne A, Shihab-Eldeen A
Department of Nuclear Medicine, Faculty of Medicine, Kuwait University, Kuwait.
Nucl Med Biol. 1996 Nov;23(8):1019-21. doi: 10.1016/s0969-8051(96)00158-8.
The effect of cyclosporine-A (CsA) on the labeling efficiencies of red blood cells with reduced 99mTcO4-; leukocytes and platelets with 111In oxine was studied. Blood was used from rats treated with CsA (30 mg/kg body weight) for 28 consecutive days and from control rats. For 99mTc labeling of RBCs, blood was obtained from individual rats and in vitro labeling technique was used. For leukocyte and platelet labeling, blood was pooled from 5 rats either treated with CsA or control. Leukocytes/platelets were labeled with 111In oxine using routine techniques. The labeling efficiency for 99mTc RBCs was 83.42 +/- 0.83% (CsA treated) and 84.85 +/- 0.62% (control); 111In-oxine leukocytes was 38.5 +/- 1.75% (CsA treated) and 42.5 +/- 3.53% (control); and for 111In-oxine platelets, it was 74.0 +/- 2.5% (CsA treated) and 78.0 +/- 1.41% (control). Comparison of the results indicate that there is no difference between the percent labeling efficiencies of 99mTc RBCs, 111In-oxine leukocytes, and 111In-oxine platelets for CsA-treated and control rats. Hence, CsA does not interfere with the labeling process of blood cells with radiopharmaceuticals.
研究了环孢素A（CsA）对用还原的99mTcO4-标记红细胞、用111In 奥辛标记白细胞和血小板的标记效率的影响。使用连续28天接受CsA（30mg/kg体重）治疗的大鼠和对照大鼠的血液。对于红细胞的99mTc标记，从个体大鼠获取血液并采用体外标记技术。对于白细胞和血小板标记，将5只接受CsA治疗或对照的大鼠的血液汇集。使用常规技术用111In 奥辛标记白细胞/血小板。99mTc红细胞的标记效率为83.42±0.83%（CsA治疗组）和84.85±0.62%（对照组）；111In-奥辛白细胞为38.5±1.75%（CsA治疗组）和42.5±3.53%（对照组）；对于111In-奥辛血小板，为74.0±2.5%（CsA治疗组）和78.0±1.41%（对照组）。结果比较表明，CsA治疗组和对照组大鼠的99mTc红细胞、111In-奥辛白细胞和111In-奥辛血小板的标记效率百分比之间没有差异。因此，CsA不干扰血细胞用放射性药物的标记过程。
