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[Automation on membranes: countercurrent isotachophoresis and immunoelectrochromatography].

作者信息

Abelev G I, Karamova E R

出版信息

Biokhimiia. 1996 Nov;61(11):1984-94.

PMID:9004859
Abstract

The review discusses two related methods--counterflow isotachophoresis and immunoaffinity electrochromatography on porous membranes developed by the authors. The methods exploit the peculiarity of electroendosmosis in porous membranes at the conditions of discontinues electrophoresis. There are described the principles and application of the above methods for automatic performance of electrophoretic analysis of low-protein biological fluids, of western blotting, epitope analysis of monoclonal antibodies and multiantigen assays. The possibility of DNA-binding protein detection is also considered.

摘要

相似文献

1
[Automation on membranes: countercurrent isotachophoresis and immunoelectrochromatography].
Biokhimiia. 1996 Nov;61(11):1984-94.
2
[Epitopic analysis of monoclonal antibodies based on countercurrent isotachophoresis on nitrocellulose membranes].[基于硝酸纤维素膜上的逆流等速电泳的单克隆抗体表位分析]
Biull Eksp Biol Med. 1988 Nov;106(11):588-90.
3
Electrochromatography: a method for automatic immunoaffinity chromatography on porous membranes.电色谱法:一种在多孔膜上进行自动免疫亲和色谱的方法。
Immunol Lett. 1994 May;40(2):133-8. doi: 10.1016/0165-2478(94)90184-8.
4
Counterflow immunoisotachophoresis on cellulose acetate membranes.醋酸纤维素膜上的对流免疫等速电泳
Anal Biochem. 1984 Nov 1;142(2):437-44. doi: 10.1016/0003-2697(84)90487-1.
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[Counter-flow isotachophoresis on cellulose acetate membranes. Role of electroendosmosis].[醋酸纤维素膜上的逆流等速电泳。电渗的作用]
Biull Eksp Biol Med. 1979 Sep;88(9):334-6.
6
[Determination of DNA-binding proteins by countercurrent isotachopheresis on nitrocellulose membranes. I. Antibodies to DNA and its adducts].[通过硝酸纤维素膜上的逆流等速电泳法测定DNA结合蛋白。I. 针对DNA及其加合物的抗体]
Mol Biol (Mosk). 1994 Jul-Aug;28(4):768-77.
7
Countercurrent immunoelectrophoresis on cellulose acetate.
J Immunol Methods. 1976;11(3-4):303-9. doi: 10.1016/0022-1759(76)90124-1.
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[Detection of blood-group antigens in objects of forensic medical expertise by electrophoresis on cellulose acetate films].
Sud Med Ekspert. 1988 Apr-Jun;31(2):21-3.
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[Countercurrent immunoblotting].[对流免疫印迹法]
Biull Eksp Biol Med. 1988 May;105(5):625-8.
10
[Use of electrophoresis on acetate cellulose film in establishing the presence and species identification of dried blood with the aid of antihemoglobin sera].[利用醋酸纤维素薄膜电泳并借助抗血红蛋白血清确定干血的存在及进行物种鉴定]
Sud Med Ekspert. 1988 Jul-Sep;31(3):31-2.

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1
Counterflow isotachophoresis as a method of concentration and isolation of DNA from biological fluids.
Dokl Biochem Biophys. 2005 May-Jun;402:200-3. doi: 10.1007/s10628-005-0070-2.