Abelev G I, Karamova E R, Lazarevich N L, Kiseleva V I, Poverenny A M
Cancer Research Center, Russian Academy of Medical Sciences, Moscow.
Immunol Lett. 1994 May;40(2):133-8. doi: 10.1016/0165-2478(94)90184-8.
Electrochromatography (ECHR) exploits a very high electro-osmotic counterflow developed in porous membranes at discontinuous electrophoresis. This counterflow exceeds considerably the anodic migration of any negatively charged protein and is used as a 'conveyer belt' for sequential transfer of immunoreagents to the specific adsorbents (antigens or antibodies) fixed on the nitrocellulose membrane. This approach was applied for simultaneous detection of two antigens (alpha-fetoprotein and carcino-embryonic antigen) in one sample, for determination of subfractions of alpha-fetoprotein, different in their epitope specificity, and for detection of L chains with certain idiotype on the background of heterogeneous L fraction. ECHR was used also for the partition of different antibodies to DNA adducts, demonstrating the possibility of applying this method to the study of DNA-binding proteins.
电色谱法（ECHR）利用在多孔膜中不连续电泳时产生的非常高的电渗逆流。这种逆流大大超过了任何带负电荷蛋白质的阳极迁移，并被用作“传送带”，将免疫试剂依次转移到固定在硝酸纤维素膜上的特异性吸附剂（抗原或抗体）上。该方法用于在一个样品中同时检测两种抗原（甲胎蛋白和癌胚抗原），测定表位特异性不同的甲胎蛋白亚组分，以及在异质轻链组分背景下检测具有特定独特型的轻链。ECHR还用于不同抗DNA加合物抗体的分离，证明了将该方法应用于研究DNA结合蛋白的可能性。
