Abelev G I, Karamova E R, Iazova A K, Gusev A I
Biull Eksp Biol Med. 1988 Nov;106(11):588-90.
A variant of the counterflow isotachophoresis on nitrocellulose membranes (NCM) was applied for the analysis of the epitope specificity of monoclonal antibodies (Mabs). Different Mabs against mouse and human alpha-fetoprotein were fixed on the membrane and the immunoreagents were transferred consequently to these dots by the electroendo-osmotic flow. The epitope specificity of the Mabs was estimated by the competition for the homologous antigen between the Mab added to the antigen and the one fixed on the membrane.
一种在硝酸纤维素膜（NCM）上的逆流等速电泳变体被用于分析单克隆抗体（Mab）的表位特异性。将针对小鼠和人甲胎蛋白的不同单克隆抗体固定在膜上，随后通过电内渗流将免疫试剂转移至这些点上。通过添加到抗原中的单克隆抗体与固定在膜上的单克隆抗体之间对同源抗原的竞争来评估单克隆抗体的表位特异性。
