[Epitopic analysis of monoclonal antibodies based on countercurrent isotachophoresis on nitrocellulose membranes].

A variant of the counterflow isotachophoresis on nitrocellulose membranes (NCM) was applied for the analysis of the epitope specificity of monoclonal antibodies (Mabs). Different Mabs against mouse and human alpha-fetoprotein were fixed on the membrane and the immunoreagents were transferred consequently to these dots by the electroendo-osmotic flow. The epitope specificity of the Mabs was estimated by the competition for the homologous antigen between the Mab added to the antigen and the one fixed on the membrane.