Londero D, Lo Greco P
Institute of Clinical Chemistry, Azienda Ospedaliera S.M.M., Udine, Italy.
J Chromatogr A. 1996 Apr 5;729(1-2):207-10. doi: 10.1016/0021-9673(95)00959-0.
An automated HPLC separation method for the detection of malondialdehyde (MDA) in plasma samples of a healthy population is described. The procedure involves a first step of acidic hydrolysis and complex formation with thiobarbituric acid (TBA), then a protein precipitation step, and finally the separation of TBA-MDA adducts through a complete HPLC apparatus with spectrofluorometric quantification. This procedure is very useful for a routine laboratory because of its rapidity (tR = 1.7 min), simplicity and applicability, and the method gives very good results with respect to linearity (0.1-12 mumol/l), precision (within-assay C.V.%: 8, between-assay C.V.%: 10) and accuracy (recovery average: 91.66%).
描述了一种用于检测健康人群血浆样本中丙二醛（MDA）的自动高效液相色谱分离方法。该程序包括第一步酸性水解并与硫代巴比妥酸（TBA）形成络合物，然后是蛋白质沉淀步骤，最后通过配备荧光光谱定量的完整高效液相色谱仪分离TBA-MDA加合物。由于其快速性（保留时间tR = 1.7分钟）、简单性和适用性，该程序对常规实验室非常有用，并且该方法在线性（0.1 - 12 μmol/l）、精密度（批内变异系数C.V.%：8，批间变异系数C.V.%：10）和准确度（平均回收率：91.66%）方面给出了非常好的结果。
