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[采用快速免疫酶法(雅培衣原体检测试剂盒)诊断泌尿生殖系统衣原体感染]

[Diagnosis of urogenital chlamydiosis using rapid immunoenzyme method: Abbott TestPack Chlamydia].

作者信息

Nazarova E K, Sozaeva L G, Shafranskiui Iu A

出版信息

Klin Lab Diagn. 1996 Sep-Oct(5):39-41.

PMID:9004993
Abstract

Twenty women suffering from chronic genital inflammations were screened for urogenital chlamydiosis using Abbott Testpack Chlamydia kit for enzyme immunoassay (EIA) and NIARmedik Chlamoscan kit for immunofluorescent method. The cells were scraped off from the cervical canal during the second phase of the cycle. In addition, smears were taken from the vagina and cervical canal to be screened for microflora using Romanowsky-Giemsa staining. EIA detected C. trachomatis antigens in 3 (15%) out of 20 examinees, whereas direct immunofluorescence revealed these antigens in only 2 (10%) patients. The results differed in a patient with urogenital candidiasis. Evidently, the system of special filters in the Testpack Chlamydia releases the cell suspension from concomitant cell dendrites, blood, mucus, and thus increases the sensitivity of the test.

摘要

使用雅培衣原体检测试剂盒进行酶免疫测定(EIA)以及NIARmedik衣原体扫描试剂盒进行免疫荧光法,对20名患有慢性生殖器炎症的女性进行了泌尿生殖系统衣原体感染筛查。在月经周期的第二阶段,从宫颈管刮取细胞。此外,从阴道和宫颈管采集涂片,使用罗曼诺夫斯基-吉姆萨染色法筛查微生物群落。酶免疫测定在20名受检者中的3名(15%)检测到沙眼衣原体抗原,而直接免疫荧光仅在2名(10%)患者中发现了这些抗原。在一名患有泌尿生殖系统念珠菌病的患者中,结果有所不同。显然,衣原体检测试剂盒中的特殊过滤系统可从伴随的细胞树突、血液、黏液中释放细胞悬液,从而提高检测的灵敏度。

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