Hayashi K, Thabit G, Massa K L, Bogdanske J J, Cooley A J, Orwin J F, Markel M D
Comparative Orthopaedic Research Laboratory, School of Veterinary Medicine, Madison, Wisconsin, USA.
Am J Sports Med. 1997 Jan-Feb;25(1):107-12. doi: 10.1177/036354659702500121.
The purpose of this study was to evaluate the effect of temperature on shrinkage and the histologic properties of glenohumeral joint capsular tissue. Six fresh-frozen cadaveric shoulders were used for this study. Seven joint capsule specimens were taken from different regions from each glenohumeral joint and assigned to one of seven treatment groups (37 degrees, 55 degrees, 60 degrees, 65 degrees, 70 degrees, 75 degrees, 80 degrees C) using a randomized block design. Specimens were placed in a tissue bath heated to one of the designated temperatures for 10 minutes. Specimens treated with temperatures at or above 65 degrees C experienced significant shrinkage compared with those treated with a 37 degrees C bath. The posttreatment lengths in the 70 degrees, 75 degrees, and 80 degrees C groups were significantly less than the pretreatment lengths. Histologic analysis revealed significant thermal alteration characterized by hyalinization of collagen in the 65 degrees, 70 degrees, 75 degrees, and 80 degrees C groups. This study demonstrated that temperatures at or above 65 degrees C caused significant shrinkage of glenohumeral joint capsular tissue. These results are consistent with histologic findings, which revealed significant thermal changes of collagen in the 65 degrees, 70 degrees, 75 degrees, and 80 degrees C groups. To verify the validity of laser application for shrinkage of joint capsule, studies designed to compare these findings with the effects of laser energy must be performed.
本研究的目的是评估温度对盂肱关节囊组织收缩及组织学特性的影响。本研究使用了六个新鲜冷冻的尸体肩部。从每个盂肱关节的不同区域获取七个关节囊标本，并采用随机区组设计将其分配到七个治疗组之一（37℃、55℃、60℃、65℃、70℃、75℃、80℃）。将标本置于加热至指定温度之一的组织浴中10分钟。与在37℃浴中处理的标本相比，在65℃及以上温度处理的标本出现了明显的收缩。70℃、75℃和80℃组的处理后长度明显小于预处理长度。组织学分析显示，65℃、70℃、75℃和80℃组出现了以胶原玻璃样变性为特征的明显热改变。本研究表明，65℃及以上的温度会导致盂肱关节囊组织明显收缩。这些结果与组织学结果一致，后者显示65℃、70℃、75℃和80℃组的胶原出现了明显的热变化。为验证激光应用于关节囊收缩的有效性，必须进行旨在将这些结果与激光能量的效果进行比较的研究。
