Han H Y, McPheron B A
Department of Entomology and Institute of Molecular Evolutionary Genetics, Pennsylvania State University, University Park 16802, USA.
Mol Phylogenet Evol. 1997 Feb;7(1):17-32. doi: 10.1006/mpev.1996.0370.
We analyzed sequence data from 925 base pairs within the mitochondrial 16S ribosomal DNA from 34 tephritid species and two outgroup taxa. DNA sequences were amplified by the polymerase chain reaction and sequenced by the dideoxy chain-termination method. Patterns of nucleotide composition and substitution were assessed based on sequence comparison. Phylogenetic relationships among the included taxa were inferred using neighbor-joining and maximum parsimony methods. The trees were congruent with the morphological classification and suggested the following phylogenetic relationships: (1) monophyly of the tribe Trypetini, s. str., excluding Parastenopa; (2) a possible sister group relationship between Platyparea and the subfamily Tephritinae; (3) a sister group relationship between Hexachaeta and the tribe Toxotrypanini; (4) monophyly of the Dacinae and possible relationship of Oedicarena to this subfamily; (5) monophyly of the tribe Adramini; and (6) the nonmonophyletic nature of the genus Rhagoletis. We propose that the 16S rDNA shows great promise for helping to create a phylogenetically based higher classification of the Tephritidae.
我们分析了34种实蝇科物种和两个外类群分类单元线粒体16S核糖体DNA中925个碱基对的序列数据。通过聚合酶链反应扩增DNA序列，并采用双脱氧链终止法进行测序。基于序列比较评估核苷酸组成和替换模式。使用邻接法和最大简约法推断所包含分类单元之间的系统发育关系。这些树状图与形态学分类一致，并表明了以下系统发育关系：（1）狭义的果实蝇族（Trypetini）是单系群，不包括副果实蝇属（Parastenopa）；（2）扁果实蝇属（Platyparea）与实蝇亚科（Tephritinae）之间可能存在姐妹群关系；（3）六鬃实蝇属（Hexachaeta）与长颜实蝇族（Toxotrypanini）之间存在姐妹群关系；（4）寡鬃实蝇亚科（Dacinae）是单系群，且油实蝇属（Oedicarena）可能与该亚科存在关系；（5）阿德拉实蝇族（Adramini）是单系群；（6）果实蝇属（Rhagoletis）的非单系性质。我们认为16S rDNA在帮助建立基于系统发育的实蝇科高级分类方面具有很大潜力。
