Prehaud C, Lopez N, Blok M J, Obry V, Bouloy M
Laboratoire des Bunyaviridés, Institut Pasteur, 25 rue du Dr Roux, Paris Cedex 15, 75 724, France.
Virology. 1997 Jan 6;227(1):189-97. doi: 10.1006/viro.1996.8324.
A reconstituted transcription system composed of the Rift Valley fever phlebovirus (Bunyaviridae family) proteins L and N expressed via recombinant vaccinia viruses and an S-like model RNA containing the CAT gene in the antisense orientation, has been described previously by Lopez et al. (J. Virol., 1995, 69, 3972-3979). We extended the use of this in vivo system to determine the sequence at the 3' end of the ambisense S segment recognized by the transcription complex. A mutational analysis of the sequences at the 3' end of the S-like genomic or antigenomic RNA was undertaken. The data indicated that the minimal sequence required for transcription resides in the 13 first 3' nucleotides of the genomic or antigenomic RNA. In these sequences, two regions appeared crucial: the bases at positions 3 to 8 and the purine at position 13. In addition, the terminal repeat ...GU could be deleted without affecting significantly the template activity of the RNA. These data support the prime and realign mechanism proposed recently for Bunya- and Arenaviruses
洛佩斯等人（《病毒学杂志》，1995年，第69卷，第3972 - 3979页）之前曾描述过一种重组转录系统，该系统由通过重组痘苗病毒表达的裂谷热静脉病毒（布尼亚病毒科）蛋白L和N以及一个含有反义方向CAT基因的S样模型RNA组成。我们扩展了这个体内系统的用途，以确定转录复合物识别的双义S片段3'端的序列。对S样基因组或反基因组RNA 3'端的序列进行了突变分析。数据表明，转录所需的最小序列位于基因组或反基因组RNA的前13个3'核苷酸中。在这些序列中，有两个区域显得至关重要：第3至8位的碱基和第13位的嘌呤。此外，末端重复序列...GU可以删除而不会显著影响RNA的模板活性。这些数据支持了最近针对布尼亚病毒和沙粒病毒提出的引物与重新排列机制。
