Natural beta-carotene and whole body irradiation in rats.

beta-carotene and other carotenoids are reported to be potent free radical quenchers, singlet oxygen scavengers, and lipid antioxidants. Whole-body irradiation is known to cause an immunosuppression effect in mammals through the possible initiation and production of reactive oxygen species. We decided to test the possible antioxidative effect against whole-body irradiation of a natural beta-carotene, composed of equal amounts of the all-trans and 9-cis isomers, obtained from the unicellular alga Dunaliella bardawil. Rats were fed on ground commercial food enriched with natural beta-carotene (50 mg/kg diet). On completion of 1 week with beta-carotene, the rats were exposed to a single dose of 4 Gy whole-body irradiation, after which their livers and blood were removed for beta-carotene and retinol analysis in comparison with control livers of animals irradiated or not, or supplemented with beta-carotene after irradiation. A normal increase in body weight with no ill effects was noted in the groups of rats whose diet was supplemented by beta-carotene before and after irradiation, compared with the reduction in the specific growth rate in the group of rats irradiated without beta-carotene. Liver beta-carotene and retinol decreased significantly after irradiation compared with the rats which were not irradiated. This decrease was not shown in rats fed beta-carotene prior to irradiation, and the effect of irradiation was partially cured by supplementation with beta-carotene after irradiation. High-pressure liquid chromatography (HPLC) analysis of the irradiated animals showed a selective decline in 9-cis beta-carotene and in retinol over all-trans beta-carotene and retinyl-esters. These results suggest that 9-cis beta-carotene and retinol protect in vivo against the cellular damage by free radicals induced after whole-body irradiation.