Ben-Amotz A, Yatziv S, Sela M, Greenberg S, Rachmilevich B, Shwarzman M, Weshler Z
Israel Oceanographic and Limnological Research, Haifa.
Radiat Environ Biophys. 1998 Oct;37(3):187-93. doi: 10.1007/s004110050116.
Attempts were made to evaluate 709 children (324 boys and 385 girls) who had been exposed long-term to different doses of radiation during and after the Chernobyl accident and had moved to Israel between 1990 and 1994. Upon arrival, all of them underwent a check-up for most common clinical disorders and were then divided into three groups according to their residences (distance from the reactor) and the level of irradiation exposure: no radiation, <5 Ci/m2, and >5 Ci/m2, respectively. Blood serum analyses for total carotenoids, retinol, alpha-tocopherol and oxidized conjugated dienes in 262 of the children showed increased HPLC levels of conjugated dienes, indicating increased levels of oxidation of in vivo blood lipids in children from the contaminated areas. The levels were higher in girls than in boys. Some 57 boys and 42 girls were given a basal diet with a diurnal supplementation of 40 mg natural 9-cis and all-trans equal isomer mixture beta-carotene in a capsulated powder form of the alga Dunaliella bardawil, for a period of 3 months. Blood serum analyses were regularly conducted before supplementation to determine the baseline effect of radiation exposure to the children, after 1 and 3 months of natural beta-carotene supplementation. After supplementation, the levels of the oxidized conjugated dienes decreased in the children's sera without any significant changes in the level of total carotenoids, retinol or alpha-tocopherol. Other common blood biochemicals were within the normal range for all tests and no statistical differences before or after supplementation of beta-carotene were noted. High pressure liquid chromatography (HPLC) analyses for carotenoids in the blood detected mainly oxycarotenoids, and to a lesser extent, all-trans beta-carotene, alpha-carotene, but not 9-cis beta-carotene. The results suggest that irradiation increases the susceptibility of lipids to oxidation in the Chernobyl children and that natural beta-carotene may act as an in vivo lipophilic antioxidant or radioprotector.
研究人员试图对709名儿童（324名男孩和385名女孩）进行评估，这些儿童在切尔诺贝利事故期间及之后长期暴露于不同剂量的辐射下，并于1990年至1994年间移居到以色列。抵达后，他们所有人都接受了最常见临床疾病的检查，然后根据其居住地点（与反应堆的距离）和辐射暴露水平分为三组：无辐射、<5 Ci/m²和>5 Ci/m²。对262名儿童的血清进行总类胡萝卜素、视黄醇、α-生育酚和氧化共轭二烯分析，结果显示共轭二烯的高效液相色谱（HPLC）水平升高，表明受污染地区儿童体内血脂的氧化水平增加。女孩的水平高于男孩。约57名男孩和42名女孩接受了基础饮食，并每日补充40毫克天然9-顺式和全反式等量异构体混合物β-胡萝卜素，以胶囊粉末形式的杜氏盐藻提供，为期3个月。在补充天然β-胡萝卜素前、补充1个月和3个月后定期进行血清分析，以确定辐射对儿童的基线影响。补充后，儿童血清中氧化共轭二烯的水平下降，而总类胡萝卜素、视黄醇或α-生育酚的水平没有任何显著变化。所有测试中其他常见血液生化指标均在正常范围内，补充β-胡萝卜素前后未发现统计学差异。血液中类胡萝卜素的高压液相色谱（HPLC）分析主要检测到氧代类胡萝卜素，较少程度检测到全反式β-胡萝卜素、α-胡萝卜素，但未检测到9-顺式β-胡萝卜素。结果表明，辐射增加了切尔诺贝利儿童脂质氧化的易感性，天然β-胡萝卜素可能作为体内亲脂性抗氧化剂或辐射防护剂发挥作用。
