无需提取的血清总3α-羟基胆汁酸浓度的比色酶法测定
Colorimetric enzymatic measurement of serum total 3 alpha-hydroxy bile acid concentrations without extraction.
作者信息
Qureshi M Y, Smith S M, Murphy G M
出版信息
J Clin Pathol. 1984 Mar;37(3):317-20. doi: 10.1136/jcp.37.3.317.
Abstract
A new method for the simpler colorimetric assay of serum total 3 alpha-hydroxy bile acid concentrations without prior extraction has been investigated. The lowest concentration that could be reliably measured was 3 mumol/l and the results obtained were in agreement with those obtained using extraction and the spectrofluorimetric enzymatic assay (y = 1.007x - 1.86, r = 0.99, n = 36). Results for 20 normal sera collected after an overnight fast were 6.2 +/- 5.1 mumol/l (mean +/- SD) and in 20 normal plasma randomly collected were 4.9 +/- 4.2 mumol/l. This new assay is comparable in specificity and sensitivity to the older spectrofluorimetric enzymatic assays and convenient for routine use.
摘要
已对一种无需预先提取即可更简便地比色测定血清总3α-羟基胆汁酸浓度的新方法进行了研究。能够可靠测量的最低浓度为3μmol/l，所得结果与采用提取法和荧光分光光度酶法获得的结果一致（y = 1.007x - 1.86，r = 0.99，n = 36）。20份过夜禁食后采集的正常血清结果为6.2±5.1μmol/l（均值±标准差），随机采集的20份正常血浆结果为4.9±4.2μmol/l。这种新测定法在特异性和灵敏度方面与较老的荧光分光光度酶法相当，且便于常规使用。
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