Krenzer K L, Freddo T F
Department of Ophthalmology, Boston University Medical Center, Massachusetts, USA.
Invest Ophthalmol Vis Sci. 1997 Jan;38(1):142-52.
To document the cytokeratin expression patterns in the normal human conjunctival epithelium obtained directly from patients using impression cytology.
Impression cytology specimens were obtained from normal volunteers using pure nitrocellulose membranes rather than cellulose acetate. The 31 volunteers of both sexes ranged in age from 18 to 79 years. Impression cytology specimens were analyzed for individual cytokeratins by either immunocytochemistry or electrophoresis with immunoblotting using a defined panel of monoclonal antibodies.
Using the corroborative methods of immunocytochemistry and electrophoresis with immunoblotting, cytokeratins characteristic of nonkeratinized, stratified (K4 and K13), simple (K8 and K19), and glandular epithelia (K7) were present in the superficial layer (s) of normal human conjunctiva. Cytokeratins typical of keratinized epithelia (K1, K2, and K10) and the keratinization-related proteins filaggrin and involucrin were not expressed in normal conjunctival epithelium.
The normal human conjunctiva demonstrates a unique cytokeratin expression pattern containing cytokeratins characteristic of nonkeratinized, stratified epithelia, as well as others more typical of a simple differentiation pattern, a glandular differentiation pattern, or both. These findings provide a foundation for examining changes in the cytokeratin expression pattern in diseased human conjunctival epithelium using impression cytology.
记录通过印片细胞学直接从患者获取的正常人结膜上皮中的细胞角蛋白表达模式。
使用纯硝酸纤维素膜而非醋酸纤维素从正常志愿者获取印片细胞学标本。31名志愿者年龄在18至79岁之间，男女皆有。使用一组特定的单克隆抗体，通过免疫细胞化学或免疫印迹电泳对印片细胞学标本中的单个细胞角蛋白进行分析。
运用免疫细胞化学和免疫印迹电泳的相互印证方法，正常人结膜表层存在非角化复层上皮（K4和K13）、单层上皮（K8和K19）以及腺上皮（K7）特有的细胞角蛋白。角化上皮典型的细胞角蛋白（K1、K2和K10）以及与角化相关的蛋白质丝聚蛋白和内披蛋白在正常结膜上皮中未表达。
正常人结膜呈现独特的细胞角蛋白表达模式，包含非角化复层上皮特有的细胞角蛋白，以及更具单层分化模式、腺分化模式或两者兼具特征的其他细胞角蛋白。这些发现为使用印片细胞学检查患病人类结膜上皮细胞角蛋白表达模式的变化奠定了基础。
