Lichtenthaler H K, Schwender J, Disch A, Rohmer M
Botanisches Institut II, Universitat Karlsruhe, Germany.
FEBS Lett. 1997 Jan 6;400(3):271-4. doi: 10.1016/s0014-5793(96)01404-4.
Isopentenyl diphosphate (IPP) is the biological C5 precursor of isoprenoids. By labeling experiments using [1-(13)C]glucose, higher plants were shown to possess two distinct biosynthetic routes for IPP biosynthesis: while the cytoplasmic sterols were formed via the acetate/mevalonate pathway, the chloroplast-bound isoprenoids (beta-carotene, lutein, prenyl chains of chlorophylls and plastoquinone-9) were synthesized via a novel IPP biosynthesis pathway (glyceraldehyde phosphate/pyruvate pathway) which was first found in eubacteria and a green alga. The dichotomy in isoprenoid biosynthesis in higher plants allows a reasonable interpretation of previous odd and inconclusive results concerning the biosynthesis of chloroplast isoprenoids, which so far had mainly been interpreted in the frame of models using compartmentation of the mevalonate pathway.
异戊烯基二磷酸（IPP）是类异戊二烯的生物C5前体。通过使用[1-(13)C]葡萄糖的标记实验表明，高等植物具有两条不同的IPP生物合成途径：细胞质中的甾醇通过乙酸/甲羟戊酸途径形成，而叶绿体结合的类异戊二烯（β-胡萝卜素、叶黄素、叶绿素的异戊烯基链和质体醌-9）则通过一种新的IPP生物合成途径（磷酸甘油醛/丙酮酸途径）合成，该途径最初是在真细菌和一种绿藻中发现的。高等植物类异戊二烯生物合成中的这种二分法能够合理地解释先前关于叶绿体类异戊二烯生物合成的奇怪且不确定的结果，迄今为止，这些结果主要是在甲羟戊酸途径区室化模型的框架内进行解释的。
