Wei Guo, Chen Yudie, Wang Jianwen, Feng Liguo
College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou, China.
PeerJ. 2024 Feb 29;12:e16929. doi: 10.7717/peerj.16929. eCollection 2024.
, a renowned ornamental plant, is cultivated for its essential oil containing valuable monoterpenes, sesquiterpenes, and other compounds widely used in the floriculture industry. Farnesyl diphosphate synthase (FPPS) is a key enzyme involved in the biosynthesis of sesquiterpenes and triterpenes for abiotic or biotic stress. In this study, we successfully cloned and characterized a full-length FPPS- encoding cDNA identified as RrFPPS1 using RT-PCR from . Phylogenetic analysis showed that RrFPPS1 belonged to the angiosperm-FPPS clade. Transcriptomic and RT-qPCR analyses revealed that the gene had tissue-specific expression patterns. Subcellular localization analysis using leaves showed that RrFPPS1 was a cytoplasmic protein. enzymatic assays combined with GC-MS analysis showed that RrFPPS1 produced farnesyl diphosphate (FPP) using isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) as substrates to provide a precursor for sesquiterpene and triterpene biosynthesis in the plant. Additionally, our research found that RrFPPS1 was upregulated under salt treatment. These substantial findings contribute to an improved understanding of terpene biosynthesis in and open new opportunities for advancements in horticultural practices and fragrance industries by overexpression of the gene increased FPP production and subsequently led to elevated sesquiterpene yields in the future. The knowledge gained from this study can potentially lead to the development of enhanced varieties of with improved aroma, medicinal properties, and resilience to environmental stressors.
[植物名称]是一种著名的观赏植物，因其精油中含有珍贵的单萜、倍半萜和其他化合物而被种植，这些化合物在花卉产业中广泛使用。法尼基二磷酸合酶（FPPS）是参与倍半萜和三萜生物合成以应对非生物或生物胁迫的关键酶。在本研究中，我们使用RT-PCR从[植物名称]成功克隆并鉴定了一个全长编码FPPS的cDNA，命名为RrFPPS1。系统发育分析表明，RrFPPS1属于被子植物-FPPS进化枝。转录组学和RT-qPCR分析显示该基因具有组织特异性表达模式。使用[植物名称]叶片进行的亚细胞定位分析表明，RrFPPS1是一种细胞质蛋白。酶促分析结合GC-MS分析表明，RrFPPS1以异戊烯基二磷酸（IPP）和二甲基烯丙基二磷酸（DMAPP）为底物产生法尼基二磷酸（FPP），为植物中倍半萜和三萜生物合成提供前体。此外，我们的研究发现RrFPPS1在盐处理下上调。这些重要发现有助于更好地理解[植物名称]中的萜类生物合成，并通过过表达该基因增加FPP产量，进而提高未来倍半萜产量，为园艺实践和香料产业的发展开辟新机遇。本研究获得的知识可能会导致培育出具有改善香气、药用特性和对环境胁迫抗性增强的[植物名称]改良品种。
