Shellard J, Perreau J, Brûlet P
Unite d'Embryologie Moleculaire, Institut Pasteur, Paris, France.
Eur Cytokine Netw. 1996 Dec;7(4):699-712.
Leukemia inhibitory factor (LIF) is a cytokine that exhibits proliferative, survival and differentiation activities on a wide range of cell types. A role for LIF in embryonic development is suggested by: i) its ability to stimulate the proliferation of embryonic stem (ES) cells in vitro, while maintaining their totipotency and ii) by both its maternal and embryonic expression at the time of blastocyst implantation. Functional studies of LIF and its receptor during mouse embryogenesis have been performed using the techniques of targeted gene replacement and transgene expression in ES cells to produce transgenic mice bearing either loss- or gain-of-function mutations for LIF activity. Whereas, the phenotype observed in the LIF gain-of-function mutant mice supports a role for LIF in early embryogenesis, the loss-of-function phenotypes point to more specialized functions for LIF in development and further reveal the redundant feature of the LIF cytokine/receptor family.
白血病抑制因子（LIF）是一种细胞因子，对多种细胞类型具有增殖、存活和分化活性。LIF在胚胎发育中的作用体现在以下方面：其一，它能够在体外刺激胚胎干细胞（ES细胞）增殖，同时维持其全能性；其二，在胚泡着床时，它既有母体表达又有胚胎表达。在小鼠胚胎发生过程中，利用靶向基因替换技术和ES细胞中的转基因表达技术，对LIF及其受体进行了功能研究，以培育出具有LIF活性功能丧失或功能获得突变的转基因小鼠。虽然在LIF功能获得突变小鼠中观察到的表型支持LIF在早期胚胎发生中的作用，但功能丧失表型则表明LIF在发育中具有更特殊的功能，并进一步揭示了LIF细胞因子/受体家族的冗余特性。
