Asano T, Kenmochi T, Isono K
Department of Surgery (II), Chiba University, School of Medicine, Japan.
Nihon Geka Gakkai Zasshi. 1996 Nov;97(11):958-63.
Organ procurement is the first step of organ preservation. We have developed "in situ machine wash out technique with CMH solution" for the purpose of rapid cooling and complete elimination of blood. The utility of this technique was confirmed by both experimentally and clinically. Two major techniques have been used for organ preservation. One is a simple cold storage. Using this method, it is easy to transport organs for transplantation. In addition, availability of UW solution is another advantage of this method. The other is a continuous hypothermic perfusion. Despite of complexity and difficulty of organ transportation, this technique has some advantages, such as viability assay and pretreatment of organs before transplantation. In our experimental studies, viability assays of canine liver and pancreas were achieved using a organ perfusion machine. We also succeeded to prolong the survival of canine pancreatic allograft by pretreatment of anti-Ia antibody during perfusion. While, cryopreservation is most suitable for cell preservation like pancreatic islets. Recently, gene technology was applied for organ preservation studies. In the future, this might become a potent tool for studies in this field.
器官获取是器官保存的第一步。我们开发了“用CMH溶液进行原位机器冲洗技术”，目的是快速降温并完全清除血液。该技术的实用性在实验和临床方面均得到了证实。目前有两种主要的器官保存技术。一种是简单的冷藏。使用这种方法，便于运输用于移植的器官。此外，UW溶液的可用性是该方法的另一个优点。另一种是持续低温灌注。尽管器官运输复杂且困难，但该技术具有一些优势，例如活力测定以及移植前对器官进行预处理。在我们的实验研究中，使用器官灌注机对犬肝脏和胰腺进行了活力测定。我们还通过在灌注过程中使用抗Ia抗体预处理，成功延长了犬胰腺同种异体移植物的存活时间。而冷冻保存最适合像胰岛这样的细胞保存。最近，基因技术被应用于器官保存研究。未来，这可能会成为该领域研究的有力工具。
