Boiadzhieva-Mikhaĭlova A, Bakalska-Nesheva M, Kancheva L, Anastasova-Kristeva M
Arkh Anat Gistol Embriol. 1977 Jul;73(7):42-6.
Changes in the ovaries of chicken embryos (17th day of incubation) and in 2-day-old chickens at different terms (2, 24, 72 h) were studied by light and electron microscopic methods after x-ray irradiation in the doses of 606 and 1200 rad. In oocytes of 17 embryos x-ray irradiation acts as an additional factor, enhancing and increasing the degree of chromatin condensation and producing earlier appearance of pachynema stage. In the ovarian oocytes, at diplonema stage, in 2-day-old chickens after irradiation, condensation of chromosomal substance is observed; in this case, irradiation produces a reverse action to that of normal despiralization. Ultrastructural changes in cellular organelles, in nuclear membrane, in particular, depend on the degree of oocyte differentiation and the irradiation dose applied. Within the terms of investigation, x-ray irradiation does not produce any considerable ultrastructural changes in the oocytes, but its disturbes the contacts between them and follicular cells, which is supposed to be the cause of oocyte destruction.
采用光学和电子显微镜方法，研究了剂量为606拉德和1200拉德的X射线照射后鸡胚（孵化第17天）以及2日龄雏鸡在不同时间（2、24、72小时）卵巢的变化。在17个胚胎的卵母细胞中，X射线照射作为一个额外因素，增强并加大了染色质凝聚程度，并使粗线期更早出现。在2日龄雏鸡处于双线期的卵巢卵母细胞中，照射后可观察到染色体物质凝聚；在这种情况下，照射产生的作用与正常解螺旋相反。细胞器尤其是核膜的超微结构变化取决于卵母细胞的分化程度和所施加的照射剂量。在研究期间，X射线照射未在卵母细胞中产生任何显著的超微结构变化，但它扰乱了卵母细胞与卵泡细胞之间的接触，这被认为是卵母细胞破坏的原因。
