Newman E A, Zahs K R
Department of Physiology, University of Minnesota, 435 Delaware Street, SE, Minneapolis, MN 55455, USA.
Science. 1997 Feb 7;275(5301):844-7. doi: 10.1126/science.275.5301.844.
Calcium signals were recorded from glial cells in acutely isolated rat retina to determine whether Ca2+ waves occur in glial cells of intact central nervous system tissue. Chemical (adenosine triphosphate), electrical, and mechanical stimulation of astrocytes initiated increases in the intracellular concentration of Ca2+ that propagated at approximately 23 micrometers per second through astrocytes and Müller cells as intercellular waves. The Ca2+ waves persisted in the absence of extracellular Ca2+ but were largely abolished by thapsigargin and intracellular heparin, indicating that Ca2+ was released from intracellular stores. The waves did not evoke changes in cell membrane potential but traveled synchronously in astrocytes and Müller cells, suggesting a functional linkage between these two types of glial cells. Such glial Ca2+ waves may constitute an extraneuronal signaling pathway in the central nervous system.
从急性分离的大鼠视网膜中的神经胶质细胞记录钙信号，以确定完整中枢神经系统组织的神经胶质细胞中是否发生Ca2+波。对星形胶质细胞进行化学（三磷酸腺苷）、电和机械刺激，可引发细胞内Ca2+浓度升高，该浓度以每秒约23微米的速度通过星形胶质细胞和米勒细胞作为细胞间波传播。Ca2+波在细胞外Ca2+缺失的情况下持续存在，但被毒胡萝卜素和细胞内肝素基本消除，表明Ca2+是从细胞内储存中释放出来的。这些波不会引起细胞膜电位的变化，但在星形胶质细胞和米勒细胞中同步传播，表明这两种神经胶质细胞之间存在功能联系。这种神经胶质细胞Ca2+波可能构成中枢神经系统中的一种神经元外信号通路。
