Irinopoulou T, Vassy J, Beil M, Nicolopoulou P, Encaoua D, Rigaut J P
Laboratory of Image Analysis in Cell Pathology, Institute of Hematology, Hospital St. Louis, University Paris 7, France.
Cytometry. 1997 Feb 1;27(2):99-105.
DNA ploidy provides important information for the evaluation of the prognosis of prostate cancer. For the purpose of DNA cytometry and nuclear measurements, we developed an image processing system for the acquisition and processing of three-dimensional (3D) images based on confocal scanning laser microscopy (CSLM). The advantage of the CSLM is the preservation of the tissue architecture and the possibility of multilabeling. It is possible to determine both individual nuclear features and cellular features and the degree of the spatial heterogeneity of several markers. Special attention was paid to the development of the automatic method for the 3D segmentation of cell nuclei. Thick tissue slides (100 microm), stained for DNA with chromomycin A3, from 4 patients (with benign hyperplasia, prostatic intraepithelial neoplasia (PIN), and well-and poorly-differentiated adenocarcinoma of the prostate), were studied in order to test the practicability of the developed methodology. DNA histograms showed a single peak in the diploid range for the hyperplasia and PIN cases. For the case of well-differentiated carcinoma, 2 peaks were observed, 1 in the diploid range and I in the tetraploid range. The case of poorly-differentiated carcinoma was characterized by an aneuploid distribution. For the cases of PIN and carcinomas, we observed a considerable variation of the volume of nuclei.
DNA倍性为评估前列腺癌的预后提供了重要信息。为了进行DNA细胞计数和细胞核测量，我们基于共聚焦扫描激光显微镜（CSLM）开发了一种用于采集和处理三维（3D）图像的图像处理系统。CSLM的优点是能够保留组织结构并进行多标记。既可以确定单个细胞核特征和细胞特征，也可以确定几种标记物的空间异质性程度。我们特别关注细胞核三维分割自动方法的开发。为了测试所开发方法的实用性，我们研究了4例患者（患有良性增生、前列腺上皮内瘤变（PIN）以及高分化和低分化前列腺腺癌）的厚组织切片（100微米），这些切片用嗜铬霉素A3对DNA进行了染色。DNA直方图显示，增生和PIN病例在二倍体范围内有一个单峰。对于高分化癌病例，观察到2个峰，1个在二倍体范围内，1个在四倍体范围内。低分化癌病例的特征是出现非整倍体分布。对于PIN和癌病例，我们观察到细胞核体积有相当大的变化。
