Irinopoulou T, Vassy J, Rigaut J P
Laboratory of Image Analysis in Cell Pathology, Hospital St. Louis, University Paris 7, France.
Anal Quant Cytol Histol. 1998 Oct;20(5):351-7.
To explore the potential of three-dimensional (3-D) image cytometry for the measurement of DNA content in prostatic specimens using confocal scanning laser microscopy (CSLM) and 3-D image analysis.
Thick tissue slices (100 microns), stained for DNA with chromomycin A3, from four patients with benign hyperplasia, prostatic intraepithelial neoplasia (PIN), well- and poorly differentiated adenocarcinoma of the prostate, were studied. Two different blocks from the same slice were studied for each case. Cell nuclei were segmented automatically. DNA content and nuclear volume were measured.
DNA histograms showed a single peak in the diploid range for the hyperplasia and PIN cases. For the case of well-differentiated carcinoma, two peaks were observed, one in the diploid range and one in the tetraploid range. The two peaks were observed on two different blocks of the same tissue slice. Poorly differentiated carcinoma was characterized by an aneuploid distribution. For the cases of PIN and carcinoma, we observed a considerable variation in nuclear volume.
The results indicate the potential of 3-D image cytometry for the measurement of DNA content in prostatic specimens while preserving tissue architecture.
利用共聚焦扫描激光显微镜（CSLM）和三维图像分析技术，探讨三维（3-D）图像细胞术测量前列腺标本中DNA含量的潜力。
对4例患有良性增生、前列腺上皮内瘤变（PIN）、高分化和低分化前列腺腺癌的患者，用放线菌素A3对其厚度为100微米的厚组织切片进行DNA染色后展开研究。每个病例研究同一切片的两个不同组织块。细胞核自动分割。测量DNA含量和核体积。
DNA直方图显示，增生和PIN病例在二倍体范围内有一个单峰。高分化癌病例观察到两个峰，一个在二倍体范围，一个在四倍体范围。这两个峰在同一组织切片的两个不同组织块上观察到。低分化癌的特征是异倍体分布。对于PIN和癌病例，我们观察到核体积有相当大的差异。
结果表明三维图像细胞术在保留组织结构的同时测量前列腺标本中DNA含量的潜力。
