神经元生长相关蛋白SCG10对微管动力学的调节
Regulation of microtubule dynamics by the neuronal growth-associated protein SCG10.
作者信息
Riederer B M, Pellier V, Antonsson B, Di Paolo G, Stimpson S A, Lütjens R, Catsicas S, Grenningloh G
机构信息
Institut de Biologie Cellulaire et de Morphologie, Lausanne, Switzerland.
出版信息
Proc Natl Acad Sci U S A. 1997 Jan 21;94(2):741-5. doi: 10.1073/pnas.94.2.741.
Abstract
Dynamic assembly and disassembly of microtubules is essential for cell division, cell movements, and intracellular transport. In the developing nervous system, microtubule dynamics play a fundamental role during neurite outgrowth, elongation, and branching, but the molecular mechanisms involved are unknown. SCG10 is a neuron-specific protein that is membrane-associated and highly enriched in growth cones. Here we show that SCG10 binds to microtubules, inhibits their assembly, and can induce microtubule disassembly. We also show that SCG10 overexpression enhances neurite outgrowth in a stably transfected neuronal cell line. These data identify SCG10 as a key regulator of neurite extension through regulation of microtubule instability.
摘要
微管的动态组装和解聚对于细胞分裂、细胞运动及细胞内运输至关重要。在发育中的神经系统里，微管动力学在神经突生长、延伸及分支过程中发挥着重要作用，但其涉及的分子机制尚不清楚。SCG10是一种神经元特异性蛋白，与细胞膜相关且在生长锥中高度富集。在此我们表明，SCG10与微管结合，抑制其组装，并能诱导微管解聚。我们还表明，在稳定转染的神经元细胞系中，SCG10的过表达可增强神经突生长。这些数据表明，SCG10是通过调节微管不稳定性来作为神经突延伸的关键调节因子。
相似文献
1
Regulation of microtubule dynamics by the neuronal growth-associated protein SCG10.神经元生长相关蛋白SCG10对微管动力学的调节
Proc Natl Acad Sci U S A. 1997 Jan 21;94(2):741-5. doi: 10.1073/pnas.94.2.741.
2
Role of the microtubule destabilizing proteins SCG10 and stathmin in neuronal growth.微管解聚蛋白SCG10和Stathmin在神经元生长中的作用。
J Neurobiol. 2004 Jan;58(1):60-9. doi: 10.1002/neu.10279.
3
SCG10, a microtubule destabilizing factor, stimulates the neurite outgrowth by modulating microtubule dynamics in rat hippocampal primary cultured neurons.SCG10是一种微管解聚因子，它通过调节大鼠海马原代培养神经元中的微管动力学来刺激神经突生长。
J Neurobiol. 2006 Sep 1;66(10):1101-14. doi: 10.1002/neu.20295.
4
The control of microtubule stability in vitro and in transfected cells by MAP1B and SCG10.微管相关蛋白1B（MAP1B）和分泌粒蛋白II（SCG10）对体外及转染细胞中微管稳定性的调控
Cell Motil Cytoskeleton. 2006 Nov;63(11):681-95. doi: 10.1002/cm.20154.
5
Calmyrin1 binds to SCG10 protein (stathmin2) to modulate neurite outgrowth.钙调素1与SCG10蛋白（即生长相关蛋白43）结合以调节神经突生长。
Biochim Biophys Acta. 2011 May;1813(5):1025-37. doi: 10.1016/j.bbamcr.2010.12.023. Epub 2011 Jan 6.
6
BRI3 associates with SCG10 and attenuates NGF-induced neurite outgrowth in PC12 cells.BRI3与SCG10相互作用，并减弱PC12细胞中神经生长因子诱导的神经突生长。
BMB Rep. 2008 Apr 30;41(4):287-93. doi: 10.5483/bmbrep.2008.41.4.287.
7
Differential distribution of stathmin and SCG10 in developing neurons in culture.培养的发育中神经元中Stathmin和SCG10的差异分布。
J Neurosci Res. 1997 Dec 15;50(6):1000-9. doi: 10.1002/(SICI)1097-4547(19971215)50:6<1000::AID-JNR10>3.0.CO;2-8.
8
Stathmin family protein SCG10 differentially regulates the plus and minus end dynamics of microtubules at steady state in vitro: implications for its role in neurite outgrowth.信号转导分子10（Stathmin家族蛋白SCG10）在体外稳态下对微管正端和负端动力学具有不同调节作用：对其在神经突生长中的作用的启示
Biochemistry. 2007 Mar 20;46(11):3543-52. doi: 10.1021/bi061819d. Epub 2007 Feb 21.
9
The interaction of RGSZ1 with SCG10 attenuates the ability of SCG10 to promote microtubule disassembly.RGSZ1与SCG10的相互作用减弱了SCG10促进微管解聚的能力。
J Biol Chem. 2002 May 17;277(20):18127-33. doi: 10.1074/jbc.M201065200. Epub 2002 Mar 6.
10
Rnd1 regulates axon extension by enhancing the microtubule destabilizing activity of SCG10.Rnd1通过增强SCG10的微管去稳定化活性来调节轴突延伸。
J Biol Chem. 2009 Jan 2;284(1):363-371. doi: 10.1074/jbc.M808126200. Epub 2008 Nov 7.
引用本文的文献
1
Stathmin-2 enhances motor axon regeneration after injury independent of its binding to tubulin.Stathmin-2可促进损伤后运动轴突的再生，且与其与微管蛋白的结合无关。
Proc Natl Acad Sci U S A. 2025 May 27;122(21):e2502294122. doi: 10.1073/pnas.2502294122. Epub 2025 May 20.
2
Tubulin Regulates the Stability and Localization of STMN2 by Binding Preferentially to Its Soluble Form.微管蛋白通过优先结合其可溶性形式来调节STMN2的稳定性和定位。
bioRxiv. 2025 Feb 28:2025.02.27.640326. doi: 10.1101/2025.02.27.640326.
3
Targeting STMN2 for neuroprotection and neuromuscular recovery in Spinal Muscular Atrophy: evidence from in vitro and in vivo SMA models.靶向STMN2用于脊髓性肌萎缩症的神经保护和神经肌肉恢复：来自体外和体内脊髓性肌萎缩症模型的证据
Cell Mol Life Sci. 2024 Dec 27;82(1):29. doi: 10.1007/s00018-024-05550-3.
4
Bi-allelic variants in WDR47 cause a complex neurodevelopmental syndrome.WDR47基因的双等位基因变异会导致一种复杂的神经发育综合征。
EMBO Mol Med. 2025 Jan;17(1):129-168. doi: 10.1038/s44321-024-00178-z. Epub 2024 Nov 28.
5
Microtubules, Membranes, and Movement: New Roles for Stathmin-2 in Axon Integrity.微管、膜和运动：Stathmin-2 在轴突完整性中的新作用。
J Neurosci Res. 2024 Sep;102(9):e25382. doi: 10.1002/jnr.25382.
6
Establishing neuronal polarity: microtubule regulation during neurite initiation.建立神经元极性：轴突起始过程中的微管调节
Oxf Open Neurosci. 2022 May 13;1:kvac007. doi: 10.1093/oons/kvac007. eCollection 2022.
7
Cryptic exon inclusion is a molecular signature of LATE-NC in aging brains.隐匿外显子包含是衰老大脑中 LATE-NC 的分子特征。
Acta Neuropathol. 2024 Feb 3;147(1):29. doi: 10.1007/s00401-023-02671-0.
8
Cryptic splicing of stathmin-2 and UNC13A mRNAs is a pathological hallmark of TDP-43-associated Alzheimer's disease.STMN2 和 UNC13A mRNA 的剪接异常是 TDP-43 相关阿尔茨海默病的病理特征。
Acta Neuropathol. 2024 Jan 4;147(1):9. doi: 10.1007/s00401-023-02655-0.
9
Stathmin-2 loss leads to neurofilament-dependent axonal collapse driving motor and sensory denervation.Stathmin-2 的缺失导致神经丝依赖性轴突崩溃，从而导致运动和感觉神经支配丧失。
Nat Neurosci. 2024 Jan;27(1):34-47. doi: 10.1038/s41593-023-01496-0. Epub 2023 Nov 23.
10
The Stathmin-2 membrane-targeting domain is required for axon protection and regulated degradation by DLK signaling.Stathmin-2 的膜靶向结构域对于轴突保护和由 DLK 信号调控的降解是必需的。
J Biol Chem. 2023 Jul;299(7):104861. doi: 10.1016/j.jbc.2023.104861. Epub 2023 May 24.
本文引用的文献
1
Expression, purification, and characterization of a highly soluble N-terminal-truncated form of the neuron-specific membrane-associated phosphoprotein SCG10.神经元特异性膜相关磷蛋白SCG10的一种高度可溶的N端截短形式的表达、纯化及特性分析
Protein Expr Purif. 1997 Mar;9(2):295-300. doi: 10.1006/prep.1996.0679.
2
SCG10 expresses growth-associated manner in developing rat brain, but shows a different pattern to p19/stathmin or GAP-43.SCG10在发育中的大鼠大脑中以生长相关的方式表达，但与p19/微管解聚蛋白或GAP-43呈现出不同的模式。
Brain Res Dev Brain Res. 1995 Dec 21;90(1-2):73-91. doi: 10.1016/0165-3806(96)83488-2.
3
The phosphoprotein stathmin is essential for nerve growth factor-stimulated differentiation.磷蛋白18对神经生长因子刺激的分化至关重要。
J Cell Biol. 1996 Jun;133(6):1383-90. doi: 10.1083/jcb.133.6.1383.
4
Microtubule stability decreases axon elongation but not axoplasm production.微管稳定性降低轴突伸长，但不影响轴浆生成。
J Neurosci. 1996 May 15;16(10):3236-46. doi: 10.1523/JNEUROSCI.16-10-03236.1996.
5
Identification of a protein that interacts with tubulin dimers and increases the catastrophe rate of microtubules.鉴定一种与微管蛋白二聚体相互作用并提高微管解聚速率的蛋白质。
Cell. 1996 Feb 23;84(4):623-31. doi: 10.1016/s0092-8674(00)81037-5.
6
Developmental mechanisms that generate precise patterns of neuronal connectivity.产生精确神经元连接模式的发育机制。
Cell. 1993 Jan;72 Suppl:77-98. doi: 10.1016/s0092-8674(05)80030-3.
7
Inhibition of axonal growth by SNAP-25 antisense oligonucleotides in vitro and in vivo.体外和体内实验中SNAP-25反义寡核苷酸对轴突生长的抑制作用。
Nature. 1993 Jul 29;364(6436):445-8. doi: 10.1038/364445a0.
8
Molecular diversity of the SCG10/stathmin gene family in the mouse.小鼠中SCG10/微管相关蛋白Stathmin基因家族的分子多样性
Genomics. 1993 Nov;18(2):360-73. doi: 10.1006/geno.1993.1477.
9
Microtubule organization and dynamics dependent on microtubule-associated proteins.微管组织和动力学依赖于微管相关蛋白。
Curr Opin Cell Biol. 1994 Feb;6(1):74-81. doi: 10.1016/0955-0674(94)90119-8.
10
Differential localization of SCG10 and p19/stathmin messenger RNAs in adult rat brain indicates distinct roles for these growth-associated proteins.大鼠成体脑内SCG10和p19/微管相关蛋白的信使核糖核酸的差异定位表明这些生长相关蛋白具有不同作用。
Neuroscience. 1994 Jun;60(4):907-26. doi: 10.1016/0306-4522(94)90271-2.
Zotero 插件