Sugiyama M, Kobori O, Atomi Y, Wada N, Kuroda A, Muto T
First Department of Surgery, Kyorin University School of Medicine, Tokyo, Japan.
Int J Pancreatol. 1996 Dec;20(3):191-6. doi: 10.1007/BF02803768.
Pancreatic exocrine hypofunction is markedly deteriorated during acute exacerbation in a rat model with chronic pancreatitis.
Little is known about pancreatic exocrine function during acute exacerbation in patients with chronic pancreatitis. We investigated changes in pancreatic exocrine function after inducing acute pancreatitis in an animal model of spontaneous chronic pancreatitis.
WBN/Kob rats with chronic pancreatitis sequentially underwent pancreatic exocrine function test 1-6 d after surgical preparation with external pancreatic fistula. We induced acute pancreatitis in another WBN/Kob rats by i.v. administration of cerulein at a rate of 10 micrograms/kg/h for 4 h 4 d after surgical preparation. Pancreatic exocrine function test was undertaken in a conscious state 1 d before and after cerulein administration.
In WBN/Kob rats not given cerulein, pancreatic exocrine function remained almost constant at 3-6 d after surgery. Marked hyperamylasemia developed immediately after cerulein administration. After its administration, the pancreas microscopically showed prominent interstitial edema and intracellular vacuolization of acinar cells in addition to the finding of pre-existing chronic pancreatitis. Basal and cholecystokinin-stimulated flow rate, bicarbonate output, and protein output, which were substantially impaired 1 d before cerulein administration, were further reduced 1 d after its administration.
在慢性胰腺炎大鼠模型中，急性加重期胰腺外分泌功能明显恶化。
关于慢性胰腺炎患者急性加重期的胰腺外分泌功能，所知甚少。我们在自发性慢性胰腺炎动物模型中诱导急性胰腺炎后，研究了胰腺外分泌功能的变化。
患有慢性胰腺炎的WBN/Kob大鼠在通过外部胰瘘进行手术准备后的1 - 6天，依次接受胰腺外分泌功能测试。我们在另一组WBN/Kob大鼠手术准备4天后，以10微克/千克/小时的速率静脉注射雨蛙素4小时，诱导其发生急性胰腺炎。在注射雨蛙素前后1天，在清醒状态下进行胰腺外分泌功能测试。
未注射雨蛙素的WBN/Kob大鼠，术后3 - 6天胰腺外分泌功能几乎保持不变。注射雨蛙素后立即出现明显的高淀粉酶血症。注射后，胰腺显微镜检查除发现先前存在的慢性胰腺炎外，还显示出明显的间质水肿和腺泡细胞内空泡化。在注射雨蛙素前1天就已严重受损的基础和胆囊收缩素刺激的流速、碳酸氢盐输出和蛋白质输出，在注射后1天进一步降低。
