Arai I, Komatsu Y, Sasaki K, Taguchi S
Central Research Laboratories, Tsumura and Co., Ibaraki, Japan.
J Gastroenterol. 1998 Apr;33(2):247-53. doi: 10.1007/s005350050078.
We compared pancreatic exocrine secretion in 5-month-old WBN/Kob rats, a model of chronic pancreatitis, with that in Wistar rats of the same age in a conscious state. Basal pancreatic secretion and pancreatic wet weight in WBN/Kob rats were lower than the values for Wistar rats. There was no difference in plasma cholecystokinin (CCK) concentration between the two types of rats. When CCK-8 was intravenously administered, the stimulation of pancreatic protein secretion in WBN/Kob rats was weaker than that in Wistar rats. When bile and pancreatic juice were diverted from the duodenum, the resulting increase in the plasma CCK concentration was similar in both types of rats, but stimulation of the volume and protein output of pancreatic juice in WBN/Kob rats was weaker than that in Wistar rats. In addition, WBN/Kob rats exhibited little increase in pancreatic wet weight because of this diversion. When secretin was intravenously administered, the stimulation of fluid secretion in WBN/ Kob rats was also weaker than that in Wistar rats. The binding of CCK-8 to pancreatic membrane fractions in WBN/Kob rats was much weaker than that in Wistar rats. Histological findings in WBN/Kob rat pancreas showed proliferation of fibrous tissue and atrophy of acinar cells. In conclusion, pancreatic exocrine secretion in response to the gastrointestinal hormones, CCK and secretin, was lower in WBN/Kob rats than in Wistar rats. These findings suggest that the hyposecretion of pancreas in WBN/Kob rats is hyporeaction of pancreatic membrane to gastrointestinal hormones.
我们将5月龄慢性胰腺炎模型WBN/Kob大鼠的胰腺外分泌与同龄清醒状态的Wistar大鼠进行了比较。WBN/Kob大鼠的基础胰腺分泌和胰腺湿重低于Wistar大鼠。两种大鼠的血浆胆囊收缩素（CCK）浓度无差异。静脉注射CCK-8时，WBN/Kob大鼠胰腺蛋白质分泌的刺激作用弱于Wistar大鼠。当胆汁和胰液从十二指肠引流时，两种大鼠血浆CCK浓度的升高相似，但WBN/Kob大鼠胰液体积和蛋白质分泌的刺激作用弱于Wistar大鼠。此外，由于这种引流，WBN/Kob大鼠的胰腺湿重几乎没有增加。静脉注射促胰液素时，WBN/Kob大鼠液体分泌的刺激作用也弱于Wistar大鼠。WBN/Kob大鼠中CCK-8与胰腺膜部分的结合比Wistar大鼠弱得多。WBN/Kob大鼠胰腺的组织学检查结果显示纤维组织增生和腺泡细胞萎缩。总之，WBN/Kob大鼠对胃肠激素CCK和促胰液素的胰腺外分泌低于Wistar大鼠。这些发现表明，WBN/Kob大鼠胰腺分泌不足是胰腺膜对胃肠激素反应低下所致。
