来自大肠杆菌的编码N-乙基马来酰亚胺还原酶的nemA基因的分子克隆。
Molecular cloning of the nemA gene encoding N-ethylmaleimide reductase from Escherichia coli.
作者信息
Miura K, Tomioka Y, Suzuki H, Yonezawa M, Hishinuma T, Mizugaki M
机构信息
Department of Pharmaceutical Sciences, Tohoku University Hospital, Aoba-ku, Sendai, Japan.
出版信息
Biol Pharm Bull. 1997 Jan;20(1):110-2. doi: 10.1248/bpb.20.110.
Using the gene mapping membrane technique, we identified a gene (nemA) that encodes N-ethylmaleimide reductase in Escherichia coli. The open reading frame encodes a polypeptide of 365 amino acids with a molecular mass of 39,514 Da. The deduced amino acid sequence showed a high degree of homology (87% identical) with the pentaerythritol tetranitrate reductase of Enterobacter cloacae and the morphinone reductase of Pseudomonas putida (52% identical).
利用基因定位膜技术，我们在大肠杆菌中鉴定出一个编码N-乙基马来酰亚胺还原酶的基因（nemA）。该开放阅读框编码一个由365个氨基酸组成的多肽，分子量为39514道尔顿。推导的氨基酸序列与阴沟肠杆菌的季戊四醇四硝酸酯还原酶和恶臭假单胞菌的吗啡酮还原酶具有高度同源性（分别为87%相同和52%相同）。
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