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乳酸发酵短杆菌的一组三个基因（dapA、orf2和dapB）编码二氢吡啶二羧酸合酶、二氢吡啶二羧酸还原酶以及功能未知的第三种多肽。

A cluster of three genes (dapA, orf2, and dapB) of Brevibacterium lactofermentum encodes dihydrodipicolinate synthase, dihydrodipicolinate reductase, and a third polypeptide of unknown function.

作者信息

Pisabarro A, Malumbres M, Mateos L M, Oguiza J A, Martín J F

机构信息

Department of Ecology, Genetics and Microbiology, Faculty of Biology, University of León, Spain.

出版信息

J Bacteriol. 1993 May;175(9):2743-9. doi: 10.1128/jb.175.9.2743-2749.1993.

DOI:10.1128/jb.175.9.2743-2749.1993
PMID:8478336
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC204578/
Abstract

The dapA and dapB genes, encoding, respectively, dihydrodipicolinate synthase and dihydrodipicolinate reductase, the two first enzymes of the lysine branch of the aspartic amino acid family, were cloned from the DNA of the amino acid-producing bacterium Brevibacterium lactofermentum. The two genes were clustered in a 3.5-kb Sau3AI-BamHI fragment but were separated by an open reading frame of 750 nucleotides. The protein encoded by this open reading frame had little similarity to any protein in the data banks, and its function remains unknown. The three genes were translated in Escherichia coli, giving the corresponding polypeptides.

摘要

分别编码二氢吡啶二羧酸合酶和二氢吡啶二羧酸还原酶（天冬氨酸氨基酸家族赖氨酸分支的头两种酶）的dapA和dapB基因，是从产氨基酸细菌乳酸发酵短杆菌的DNA中克隆出来的。这两个基因聚集在一个3.5kb的Sau3AI - BamHI片段中，但被一个750个核苷酸的开放阅读框隔开。这个开放阅读框编码的蛋白质与数据库中的任何蛋白质几乎没有相似性，其功能仍然未知。这三个基因在大肠杆菌中进行了翻译，产生了相应的多肽。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7d0/204578/527a82b5d5e7/jbacter00051-0276-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7d0/204578/527a82b5d5e7/jbacter00051-0276-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7d0/204578/527a82b5d5e7/jbacter00051-0276-a.jpg

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本文引用的文献

1
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Appl Environ Microbiol. 1991 Jun;57(6):1746-1752. doi: 10.1128/aem.57.6.1746-1752.1991.
2
The codon preference plot: graphic analysis of protein coding sequences and prediction of gene expression.密码子偏好性图：蛋白质编码序列的图形分析及基因表达预测
Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):539-49. doi: 10.1093/nar/12.1part2.539.
3
A rapid boiling method for the preparation of bacterial plasmids.
J Microbiol. 2011 Dec;49(6):974-80. doi: 10.1007/s12275-011-1507-1. Epub 2011 Dec 28.
4
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5
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6
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Proc Natl Acad Sci U S A. 1996 Mar 5;93(5):1962-6. doi: 10.1073/pnas.93.5.1962.
7
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Proc Natl Acad Sci U S A. 1996 Mar 5;93(5):1956-61. doi: 10.1073/pnas.93.5.1956.
8
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9
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Antonie Van Leeuwenhoek. 1993;64(2):145-63. doi: 10.1007/BF00873024.
10
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J Bacteriol. 1994 Jul;176(14):4424-9. doi: 10.1128/jb.176.14.4424-4429.1994.
一种制备细菌质粒的快速煮沸法。
Anal Biochem. 1981 Jun;114(1):193-7. doi: 10.1016/0003-2697(81)90473-5.
4
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J Biol Chem. 1984 Dec 10;259(23):14829-34.
5
The condensation step in diaminopimelate synthesis.二氨基庚二酸合成中的缩合步骤。
J Biol Chem. 1965 Dec;240(12):4710-6.
6
Genetic analysis of diaminopimelic acid- and lysine-requiring mutants of Escherichia coli.大肠杆菌中对二氨基庚二酸和赖氨酸有需求的突变体的遗传分析。
J Bacteriol. 1971 Mar;105(3):844-54. doi: 10.1128/jb.105.3.844-854.1971.
7
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J Mol Biol. 1986 Jan 5;187(1):101-7. doi: 10.1016/0022-2836(86)90409-2.
8
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Biochem Biophys Res Commun. 1985 Dec 31;133(3):1013-9. doi: 10.1016/0006-291x(85)91237-9.
9
Complete nucleotide sequence of the E. coli N-acetylneuraminate lyase.大肠杆菌N-乙酰神经氨酸裂解酶的完整核苷酸序列。
Nucleic Acids Res. 1985 Dec 20;13(24):8843-52. doi: 10.1093/nar/13.24.8843.
10
Chromosomal location and nucleotide sequence of the Escherichia coli dapA gene.大肠杆菌dapA基因的染色体定位和核苷酸序列。
J Bacteriol. 1986 Apr;166(1):297-300. doi: 10.1128/jb.166.1.297-300.1986.