Vartak S, George K C, Singh B B
Radiation Biology and Biochemistry Division, Bhabha Atomic Research Centre, Bombay, India.
Indian J Exp Biol. 1996 Sep;34(9):825-32.
Antitumor effects of pre-transplantation hyperthermia and its correlation to immunological changes in the host animals and enhancement by dietary unsaturated fat have been studied. Leg muscles of mice were locally heated (41 degrees-43 degrees C, 40 min) and fibrosarcoma cells were inoculated into the heated and unheated sites and tumor growth time was determined. Spleen cell activity and plasma levels of interleukins (IL1 and 2) [RIA kits] were assessed. Muscle and tumor fatty acid profiles were modified by feeding mice with unsaturated fat supplemented diet and analysed by GLC. Pre-transplantation hyperthermia suppressed the tumor growth on both heated and unheated contralateral legs of mice. These effects were associated with increased spleen cell activity and plasma levels of IL1 and 2. Diet rich in unsaturated fat altered the fatty acid profiles of the leg muscles and tumors and inhibited tumor growth. It also potentiated the antitumor effect of pre-transplantation hyperthermia. In conclusion, mild local hyperthermia induces both direct and abscopal antitumor effects which may be ascribed to stimulation of host's antitumor immune responses and these effects are augmented by unsaturated fat supplemented diet.
已对移植前热疗的抗肿瘤作用及其与宿主动物免疫变化的相关性以及膳食不饱和脂肪的增强作用进行了研究。将小鼠腿部肌肉局部加热（41摄氏度至43摄氏度，40分钟），然后将纤维肉瘤细胞接种到加热和未加热部位，并测定肿瘤生长时间。评估脾细胞活性和白细胞介素（IL1和IL2）的血浆水平[放射免疫分析试剂盒]。通过给小鼠喂食补充不饱和脂肪的饮食来改变肌肉和肿瘤的脂肪酸谱，并通过气相色谱法进行分析。移植前热疗抑制了小鼠加热和未加热对侧腿部的肿瘤生长。这些作用与脾细胞活性增加以及IL1和IL2的血浆水平升高有关。富含不饱和脂肪的饮食改变了腿部肌肉和肿瘤的脂肪酸谱并抑制了肿瘤生长。它还增强了移植前热疗的抗肿瘤作用。总之，轻度局部热疗可诱导直接和远隔抗肿瘤作用，这可能归因于对宿主抗肿瘤免疫反应的刺激，并且这些作用通过补充不饱和脂肪的饮食而增强。
