Crittenden S L, Rudel D, Binder J, Evans T C, Kimble J
Howard Hughes Medical Institute, University of Wisconsin-Madison 53706, USA.
Dev Biol. 1997 Jan 1;181(1):36-46. doi: 10.1006/dbio.1996.8413.
The translation of maternal glp-1 mRNA is regulated both temporally and spatially in the early Caenorhabditis elegans embryo (T. C. Evans, S. L. Crittenden, V. Kodoyianni, and J. Kimble, Cell 77, 183-194, 1994). To investigate the control of embryonic glp-1 expression, we have examined the distribution of GLP-1 protein in selected maternal effect mutants that affect pattern or fate in the early embryo. We find that mutants that disrupt anterior-posterior asymmetry in the early embryo (par-1-par-6, emb-8, Par(q537)) disrupt the spatial but not temporal control of GLP-1 expression: GLP-1 is observed at the normal stage of embryogenesis in par-like mutants; however, it is uniformly distributed. In contrast, mutants that alter blastomere identity (skn-1, pie-1, mex-1, apx-1) do not affect the normal GLP-1 pattern. We conclude that genes controlling the asymmetry of cellular components, including P granules, also control GLP-1 asymmetry in the early embryo. The finding that mutants that disrupt anterior-posterior asymmetry translate GLP-1 in all blastomeres suggests that loss of embryonic asymmetry causes translational activation of GLP-1 in the posterior.
在秀丽隐杆线虫早期胚胎中，母体glp - 1 mRNA的翻译在时间和空间上均受到调控（T. C. 埃文斯、S. L. 克里滕登、V. 科多扬尼和J. 金布尔，《细胞》77卷，183 - 194页，1994年）。为了研究胚胎期glp - 1表达的调控机制，我们检测了GLP - 1蛋白在一些影响早期胚胎模式或命运的母体效应突变体中的分布情况。我们发现，那些破坏早期胚胎前后不对称性的突变体（par - 1 - par - 6、emb - 8、Par(q537)）会破坏GLP - 1表达的空间调控，但不影响时间调控：在par样突变体的胚胎发育正常阶段可观察到GLP - 1；然而，它呈均匀分布。相比之下，那些改变卵裂球特性的突变体（skn - 1、pie - 1、mex - 1、apx - 1）并不影响GLP - 1的正常模式。我们得出结论，控制包括P颗粒在内的细胞成分不对称性的基因，也控制早期胚胎中GLP - 1的不对称性。破坏前后不对称性的突变体在所有卵裂球中都能翻译GLP - 1这一发现表明，胚胎不对称性的丧失会导致GLP - 1在后部的翻译激活。
