Nakano T, Sim J S, Imai S, Koga T
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Canada.
Histochem J. 1996 Dec;28(12):867-73. doi: 10.1007/BF02331390.
Monoclonal antibodies specific to chondroitin sulphate (CS-56) and keratan sulphate (AH12) were used to localize proteoglycans in the proximal tibial articular cartilage and growth plate of broiler chickens. There was no CS-56 labelling in the proliferative zone of the growth plate. In contrast, intense labelling with this antibody was observed in the transitional and hypertrophic zones of the growth plate and the articular cartilage. This was confirmed by extracting chondroitin sulphate fractions from different zones of the growth plate and articular cartilage, and examining their antigenicities to CS-56 by ELISA inhibition assay. It was suggested that the maturation of chondrocytes in the growth plate is related to the production of chondroitin sulphate with CS-56 epitope, which may be a prerequisite for normal endochondral bone formation in the chicken tibia. The role of chondroitin sulphate recognized by CS-56 in the articular cartilage is unknown.
使用针对硫酸软骨素（CS-56）和硫酸角质素（AH12）的单克隆抗体来定位肉鸡胫骨近端关节软骨和生长板中的蛋白聚糖。生长板的增殖区没有CS-56标记。相比之下，在生长板的过渡区和肥大区以及关节软骨中观察到该抗体的强烈标记。通过从生长板和关节软骨的不同区域提取硫酸软骨素组分，并通过ELISA抑制试验检测它们对CS-56的抗原性，证实了这一点。有人提出，生长板中软骨细胞的成熟与具有CS-56表位的硫酸软骨素的产生有关，这可能是鸡胫骨正常软骨内成骨的先决条件。CS-56识别的硫酸软骨素在关节软骨中的作用尚不清楚。
