Sirek O V, Sirek A, Fikar K
Atherosclerosis. 1977 Jun;27(2):227-33. doi: 10.1016/0021-9150(77)90060-0.
Hyaluronate (HA), heparan sulfate (HS), dermatan sulfate (DS) and isomeric chondroitin sulfates (CS) were measured in vascular walls of 9-10 months old normal and hypophysectomized female beagles treated with sex hormones. Following hypophysectomy the animals were maintained for 8 weeks without any hormonal replacement therapy and then they were exposed for 3 weeks to parenteral treatment with sex hormones. One group received twice weekly 25 mg testosterone, another group was given the same amount of progesterone, and a third group received on day 1 and day 14, estrogens in 2 injections, consisting of a mixture of 10 mg short-acting estradiol-17-phenylpropionate and 2.5 mg long-acting estradiol benzoate. After 11 weeks all animals were sacrificed, coronary arteries and aortas were immediately removed and the latter were divided into three segments: arch, thoracic and abdominal. Removal of the pituitary led to a reduction of the HA content in the aortic arch and thoracic segment, but coronary arteries and abdominal aorta were not affected. The main consequence of hypophysectomy both in the entire aorta and in coronary arteries was a sharp reduction of the sulfated glycosaminoglycan (GAG) content. All three hormones produced a modest rise in the HS content of coronary arteries. A more definite response was seen in the thoracic aorta where each of the three hormones raised the low DS content to normal levels. Concerning the effect of sex hromones on aortic GAG other than DS, TESTOSTERONE RAISED THE CS content towards normal in thoracic and abdominal segments, while estrogen by doubling the normal HA concentration was particularly potent in the abdominal aorta. It is conceivable that the different sensitivity of various segments of aorta and coronary arteries to sex (and other) hormones in terms of regulating GAG metabolism may prove to be of relevance to the uneven distribution of lesions in degenerative vascular disease.
在接受性激素治疗的9 - 10月龄正常和垂体切除的雌性比格犬的血管壁中，检测了透明质酸（HA）、硫酸乙酰肝素（HS）、硫酸皮肤素（DS）和异构硫酸软骨素（CS）。垂体切除后，动物在无任何激素替代治疗的情况下维持8周，然后接受3周的性激素肠胃外治疗。一组每周两次接受25mg睾酮，另一组给予相同剂量的孕酮，第三组在第1天和第14天接受两次雌激素注射，由10mg短效17 - 苯丙酸雌二醇和2.5mg长效苯甲酸雌二醇的混合物组成。11周后，所有动物被处死，立即取出冠状动脉和主动脉，后者被分为三段：弓部、胸部和腹部。垂体切除导致主动脉弓和胸段HA含量降低，但冠状动脉和腹主动脉未受影响。垂体切除在整个主动脉和冠状动脉中的主要后果是硫酸化糖胺聚糖（GAG）含量急剧降低。所有三种激素都使冠状动脉的HS含量适度升高。在胸主动脉中观察到更明确的反应，三种激素中的每一种都将低水平的DS含量提高到正常水平。关于性激素对除DS以外的主动脉GAG的影响，睾酮使胸段和腹段的CS含量恢复正常，而雌激素通过使正常HA浓度加倍，在腹主动脉中作用尤为显著。可以想象，主动脉和冠状动脉各段在调节GAG代谢方面对性激素（及其他激素）的不同敏感性，可能与退行性血管疾病中病变的不均匀分布有关。
