Salazar N M, Caetano-Anollés G
Digital Diagnostica S.R.L., Maldonado, Uruguay.
Nucleic Acids Res. 1996 Dec 15;24(24):5056-7. doi: 10.1093/nar/24.24.5056.
Nucleic acid scanning by hybridization (NASBH) is a non-electrophoretic typing strategy that uses gridded oligonucleotides to reproducibly characterize arbitrarily amplified nucleic acid sequences. Membrane-bound arrays of terminally-degenerate oligonucleotides were hybridized to DNA amplification fingerprinting (DAF) products from enterohemorrhagic Escherichia coli O157:H7 isolates. Numerical and cluster analysis of 64 isolates, selected by DAF to represent a single dominant amplification type identified 14 hybridization types. Results show that NASBH is a powerful alternative for the identification of closely related bacteria, can be used successfully in epidemiological studies, and holds potential in general nucleic acid diagnostics.
通过杂交进行核酸扫描（NASBH）是一种非电泳分型策略，它使用网格化寡核苷酸来可重复地表征任意扩增的核酸序列。将末端简并寡核苷酸的膜结合阵列与来自肠出血性大肠杆菌O157:H7分离株的DNA扩增指纹图谱（DAF）产物进行杂交。通过DAF选择的64株代表单一优势扩增类型的分离株，经数值和聚类分析确定了14种杂交类型。结果表明，NASBH是鉴定密切相关细菌的有力替代方法，可成功用于流行病学研究，并且在一般核酸诊断中具有潜力。
