Loeffler D A, Juneau P L, Nguyen H U, Najman D, Pomara N, LeWitt P A
Neuroscience Program, Sinai Hospital, Detroit, Michigan 48235, USA.
Neurochem Res. 1997 Feb;22(2):209-14. doi: 10.1023/a:1027323809229.
Immunocytochemical staining was performed to investigate the presence of anti-hippocampal antibodies in cerebrospinal fluid (CSF) from patients with probable Alzheimer's disease (AD) (n = 19), aged normal controls (n = 9), and young normal controls (n = 10). Marked staining of neurons in the granule cell layer of the dentate gyrus and in pyramidal neurons in CA1-3 of the rat hippocampus was observed in 5 AD CSF samples (26%), 1 aged control sample (11%), and 1 young control sample (10%). These differences were not statistically significant. One of the immunoreactive AD CSF specimens also contained high concentrations of C5b-9, the membrane attack complex. The infrequent occurrence of anti-hippocampal antibodies in AD CSF, and the detection of similar immunoreactivity in control CSF specimens, suggest that these antibodies are unlikely to play a role in the neurodegenerative process in most individuals with AD. However, elevated C5b-9 concentration in an AD CSF specimen with marked immunoreactivity to hippocampal neurons suggests the possibility that anti-neuronal antibodies may contribute to complement activation in some AD patients.
进行免疫细胞化学染色，以研究可能患有阿尔茨海默病（AD）的患者（n = 19）、老年正常对照者（n = 9）和年轻正常对照者（n = 10）的脑脊液（CSF）中抗海马抗体的存在情况。在5份AD脑脊液样本（26%）、1份老年对照样本（11%）和1份年轻对照样本（10%）中，观察到大鼠海马齿状回颗粒细胞层的神经元以及CA1-3区锥体细胞有明显染色。这些差异无统计学意义。其中一份具有免疫反应性的AD脑脊液标本还含有高浓度的膜攻击复合物C5b-9。AD脑脊液中抗海马抗体的出现频率较低，且在对照脑脊液标本中检测到类似的免疫反应性，这表明这些抗体在大多数AD患者的神经退行性过程中不太可能起作用。然而，一份对海马神经元有明显免疫反应性的AD脑脊液标本中C5b-9浓度升高，提示抗神经元抗体可能在某些AD患者中促成补体激活的可能性。
