Lee S M, Lin S Y, Cheng C L, Liang R C
Department of Ophthalmology, Veterans General Hospital-Taipei, Taiwan, Republic of China.
Acta Ophthalmol Scand. 1996 Dec;74(6):542-6. doi: 10.1111/j.1600-0420.1996.tb00731.x.
The lens capsules of senile cataractous patients were differentiated into three progressive grades of immature (I, II and III) and two progressive grades of mature (I' and II') groups using Fourier transform infrared (FT-IR) microspectroscopy with Fourier self-deconvolution and curve-fitting algorithms, according to the changes in IR peak position and its structural composition. The secondary conformation of both immature-II and mature-I' cataractous human lens capsules was found to change significantly when enhancing the beta-sheet structure but simultaneously decreasing the beta-turn structures, as compared with the composition of normal human and immature-I cataractous lens capsules. The increase in beta-sheet structural proportion might possibly be attributable to the age-related cataractogenesis. We also found that the IR peak at 1651 cm-1 assigned to a-helix shifted to 1647 cm-1 that corresponded to disordered structure, but the IR peak at 1662 cm-1 due to beta-turn structure disappeared and another new IR peak assigned to alpha-helix structure appeared at 1656 cm-1, for both immature-III and mature-II' cataractous lens capsules. It increased the compositions of beta-sheet and disorder structures and simultaneously decreased the triple helix and beta-turn structures, but maintained the same level in alpha-helix structure. This suggests that FT-IR microspectroscopy can act as a potential tool to exactly differentiate the maturity of senile cataractous human lens capsules according to the changes in IR peak position and compositions in amide I band.
利用傅里叶变换红外（FT-IR）显微光谱技术结合傅里叶自去卷积和曲线拟合算法，根据红外峰位置及其结构组成的变化，将老年性白内障患者的晶状体囊膜分为三个渐进的未成熟等级（I、II和III）和两个渐进的成熟等级（I'和II'）组。与正常人和未成熟I期白内障晶状体囊膜的组成相比，当增强β-折叠结构但同时减少β-转角结构时，未成熟II期和成熟I'期白内障人晶状体囊膜的二级构象发生了显著变化。β-折叠结构比例的增加可能归因于年龄相关性白内障的发生。我们还发现，对于未成熟III期和成熟II'期白内障晶状体囊膜，归属于α-螺旋的1651 cm-1处的红外峰移至对应无序结构的1647 cm-1处，而由于β-转角结构产生的1662 cm-1处的红外峰消失，在1656 cm-1处出现了另一个归属于α-螺旋结构的新红外峰。它增加了β-折叠和无序结构的组成，同时减少了三螺旋和β-转角结构，但α-螺旋结构保持在相同水平。这表明FT-IR显微光谱技术可作为一种潜在工具，根据酰胺I带中红外峰位置和组成的变化，准确区分老年性白内障人晶状体囊膜的成熟度。
