Lin S Y, Lee S M, Li M J, Liang R C
Department of Medical Research and Education, Veterans General Hospital-Taipei, People's Republic of China.
Spectrochim Acta A Mol Biomol Spectrosc. 1997 Aug;53A(9):1507-13. doi: 10.1016/s1386-1425(97)00077-2.
The possible changes in protein structures of the cataractous human lens capsules of the immature patients with myopia and/or systemic hypertension have been investigated using Fourier transform infrared (FT-IR) microspectroscopy. Second-derivative and deconvolution methods have been applied to obtain the position of the overlapping components of the amide I band and assign them to different secondary structures. Changes in the protein secondary structure and composition of amide I band were estimated quantitatively from Fourier self-deconvolution and curve fitting algorithms. The results indicate that myopia and/or systemic hypertension were found to significantly modify the protein secondary structure of the cataractous human lens capsules to increase the beta-type structure and random coil and decrease the alpha-helix structure. Myopia-induced conformational change in triple helix structure was more pronounced. In conclusion, myopia and/or systemic hypertension seem to modify the conformation of the protein structures in cataractous human lens capsule to change ionic permeation through lens capsule to accelerate the cataract formation of senile patients.
利用傅里叶变换红外（FT-IR）显微光谱技术，研究了患有近视和/或系统性高血压的未成熟患者白内障人类晶状体囊膜蛋白质结构的可能变化。应用二阶导数和去卷积方法来获取酰胺I带重叠成分的位置，并将它们归属于不同的二级结构。通过傅里叶自去卷积和曲线拟合算法定量估计了蛋白质二级结构和酰胺I带组成的变化。结果表明，近视和/或系统性高血压显著改变了白内障人类晶状体囊膜的蛋白质二级结构，增加了β型结构和无规卷曲，减少了α-螺旋结构。近视引起的三螺旋结构构象变化更为明显。总之，近视和/或系统性高血压似乎会改变白内障人类晶状体囊膜中蛋白质结构的构象，从而改变晶状体囊膜的离子渗透，加速老年患者白内障的形成。
