Köhn F M, Schroeder-Printzen I, el Mulla K F, Jung A, Stalf T, Weidner W, Gips H, Künzel W, Schill W B
Center of Dermatology and Andrology, Justus Liebig University Giessen, Germany.
Andrologia. 1996;28 Suppl 1:77-81.
Microsurgical epididymal sperm aspiration (MESA) and testicular sperm extraction (TESE) require close cooperation between andrologists, urologists and gynaecologists. Intracytoplasmic sperm injections were established in Giessen in March 1994 and embryo transfer (ET) was performed in 342 of 375 patients (91.2%). The percentage of pregnancies and ongoing pregnancies are 35.4% per ET (32.3% per cycle) and 25.1% per ET (22.9% per cycle), respectively. Microsurgical procedures such as epididymovasostomy or vasovasostomy and cryopreservation of human semen are also established methods. The purpose of the present study was to describe the andrological work-up for patients before MESA and TESE. Experiments demonstrate that incubation of testicular tissue samples in IVF medium and treatment with 1 mg ml-1 pentoxifylline increase the number of extracted motile spermatozoa. Centrifugation of the medium results in a further concentration of sperm cells. If no motile spermatozoa can be found in the supernatant medium, they may be extracted directly from the testicular tissue samples by means of a micromanipulator.
显微外科附睾精子抽吸术（MESA）和睾丸精子提取术（TESE）需要男科医生、泌尿科医生和妇科医生密切合作。1994年3月在吉森开展了卵胞浆内单精子注射术，375例患者中有342例（91.2%）进行了胚胎移植（ET）。妊娠率和持续妊娠率分别为每次ET 35.4%（每个周期32.3%）和每次ET 25.1%（每个周期22.9%）。显微外科手术如附睾输精管吻合术或输精管吻合术以及人类精液冷冻保存也是成熟的方法。本研究的目的是描述MESA和TESE术前患者的男科检查情况。实验表明，将睾丸组织样本在体外受精培养基中孵育并用1毫克/毫升己酮可可碱处理可增加提取的活动精子数量。培养基离心可进一步浓缩精子细胞。如果在上清液培养基中未发现活动精子，可通过显微操作器直接从睾丸组织样本中提取精子。
