Orzechowska B, Błach-Olszewska Z
Laboratory of Virology, Polish Academy of Sciences, Wroclaw, Poland.
Arch Immunol Ther Exp (Warsz). 1996;44(5-6):325-8.
We have studied the effect of culturing in vitro of BALB/c--mouse resident peritoneal cells (RPC) on their ability to replicate vesicular stomatitis virus (VSV). Results of 36 experiments performed on RPC, freshly isolated from individual female mice, showed that over 70% of them expressed constitutive antiviral immunity against VSV infection. The virus multiplied to low titers (< or = 10(3) TCID50/10(6) cells) only in 10 out of 36 cases. After several days in culture, the RPC were found to lose the resistance and VSV multiplied in the cells to high titers (up to 10(7) TCID50/10(6) cells). The time period, required for the cultures to reach a full ability to replicate the virus, was individually differentiated and ranged from 24 h to over 96 h.
我们研究了体外培养BALB/c小鼠腹腔常驻细胞（RPC）对其复制水疱性口炎病毒（VSV）能力的影响。对从个体雌性小鼠新鲜分离的RPC进行的36次实验结果表明，超过70%的RPC对VSV感染表现出组成性抗病毒免疫力。在36例中，只有10例病毒增殖至低滴度（≤10³TCID50/10⁶细胞）。培养几天后，发现RPC失去抗性，VSV在细胞中增殖至高滴度（高达10⁷TCID50/10⁶细胞）。培养物达到完全复制病毒能力所需的时间段存在个体差异，范围从24小时到超过96小时。
