Orzechowska B, Janusz M, Domaraczenko B, Błach-Olszewska Z
Laboratory of Virology, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland.
Acta Virol. 1998 Apr;42(2):75-8.
It is known that resident peritoneal (RP) cells from BALB/c female mice express a constitutive non-specific antiviral immunity which is progressively reduced during several days of cultivation in vitro. In this report, we have studied the effect of a proline-rich polypeptide (PRP) isolated from ovine colostrum on the kinetics of vesicular stomatitis virus (VSV) replication in freshly isolated and one-day cultured RP cells. The polypeptide was added to the cells immediately after virus adsorption or one day before or after viral infection. Independently on time of PRP addition, an inhibition of VSV replication (virus titres reduced by up to 4 log units) was observed. Occasionally, however, a weak stimulation of VSV replication by PRP (virus titres increased by 1-2 log units) was noticed in RP cells constitutively resistant to the infection.
已知来自BALB/c雌性小鼠的腹膜常驻（RP）细胞表达一种组成性非特异性抗病毒免疫，该免疫在体外培养数天期间会逐渐降低。在本报告中，我们研究了从羊初乳中分离出的富含脯氨酸的多肽（PRP）对水泡性口炎病毒（VSV）在新鲜分离的和培养一天的RP细胞中复制动力学的影响。在病毒吸附后立即或在病毒感染前一天或后一天将该多肽添加到细胞中。无论PRP添加时间如何，均观察到VSV复制受到抑制（病毒滴度降低多达4个对数单位）。然而，偶尔在对感染具有组成性抗性的RP细胞中会注意到PRP对VSV复制有微弱刺激（病毒滴度增加1 - 2个对数单位）。
