介导mod(mdg4)突变沉默效应的su(Hw)蛋白功能结构域的表征。
Characterization of functional domains of the su(Hw) protein that mediate the silencing effect of mod(mdg4) mutations.
作者信息
Gdula D A, Corces V G
机构信息
Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218, USA.
出版信息
Genetics. 1997 Jan;145(1):153-61. doi: 10.1093/genetics/145.1.153.
Abstract
The suppressor of Hairy-wing [su(Hw)] protein represses enhancer function in a unidirectional fashion: enhancers segregated from the promoter by the su(Hw) binding region are rendered inactive. whereas those in the same domain are unaffected. In the case of the gypsy-induced y2 allele, the repressive effect of su(Hw) is rendered bidirectional in mod(mdg4) mutant flies, and all enhancers of the affected gene become inactive. This silencing of enhancer elements might be due to exposure of specific domains of su(Hw) when the mod(mdg4) protein is absent. Two of three regions of su(Hw) that are located adjacent to the leucine zipper motif and are conserved across Drosophila species are necessary for both the unidirectional and bidirectional repression of transcription by su(Hw). In contrast, two acidic domains that are dispensable for the unidirectional repression of enhancer elements are critical for the bidirectional silencing of enhancer activity observed in mutants lacking functional mod(mdg4) protein.
摘要
毛翅抑制蛋白[su(Hw)]以单向方式抑制增强子功能：被su(Hw)结合区域与启动子分隔开的增强子会失去活性，而处于同一区域的增强子则不受影响。在gypsy诱导的y2等位基因的情况下，在mod(mdg4)突变果蝇中，su(Hw)的抑制作用变为双向，受影响基因的所有增强子都失去活性。增强子元件的这种沉默可能是由于在缺少mod(mdg4)蛋白时su(Hw)特定结构域的暴露。su(Hw)中与亮氨酸拉链基序相邻且在果蝇物种中保守的三个区域中的两个，对于su(Hw)对转录的单向和双向抑制都是必需的。相比之下，对于增强子元件单向抑制可有可无的两个酸性结构域，对于在缺乏功能性mod(mdg4)蛋白的突变体中观察到的增强子活性双向沉默至关重要。
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本文引用的文献
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Interaction between mutations in the suppressor of Hairy wing and modifier of mdg4 genes of Drosophila melanogaster affecting the phenotype of gypsy-induced mutations.黑腹果蝇毛翅抑制基因和mdg4修饰基因的突变之间的相互作用影响了吉普赛诱导突变的表型。
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The su(Hw) protein insulates expression of the Drosophila melanogaster white gene from chromosomal position-effects.抑制子（Hairy-wing）蛋白使黑腹果蝇白色基因的表达免受染色体位置效应的影响。
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The su(Hw) protein bound to gypsy sequences in one chromosome can repress enhancer-promoter interactions in the paired gene located in the other homolog.与一条染色体上的“gypsy”序列结合的“su(Hw)”蛋白，可以抑制位于另一条同源染色体上的配对基因中的增强子-启动子相互作用。
Proc Natl Acad Sci U S A. 1995 May 23;92(11):5184-8. doi: 10.1073/pnas.92.11.5184.
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A Drosophila protein that imparts directionality on a chromatin insulator is an enhancer of position-effect variegation.一种赋予染色质绝缘子方向性的果蝇蛋白质是位置效应斑驳的增强子。
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Chromatin insulators. Keeping enhancers under control.染色质绝缘子：控制增强子
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