Selvaag E
Department of Dermatology, Ullevaal Hospital, University of Oslo, Norway.
Photodermatol Photoimmunol Photomed. 1996 Aug;12(4):166-70. doi: 10.1111/j.1600-0781.1996.tb00194.x.
The sulphonamide-derived oral antidiabetic drugs carbutamide, chlorpropamide, glibenclamide, glibornuride, gliclazide, glipizide, gliquidone, glisoxepide, glymidine, tolazamide and tolbutamide were investigated for photohemolytic properties in vitro. Irradiation with a SOL 3 apparatus (solar simulating irradiation) revealed hemolysis in the presence of chlorpropamide, glipizide, gliquidone, glymidine and tolbutamide (all in the concentration 10(-3) mol/l). Except for glymidine, which exerted photohemolysis in the concentration 10(-4) mol/l, no hemolytic effects were seen in the concentration of 10(-4) mol/l or 10(-5) mol/l. Irradiation with TL 12 light bulbs (UVB), a UVASUN 5000 apparatus (UVA) or an experimental lamp (visible light) did not induce phototoxic hemolysis with either of the test substances. Addition of the antioxidants ascorbic acid, alpha-tocopherol or superoxide dismutase significantly inhibited the phototoxic hemolysis. Investigations carried out in a nitrogen-rich atmosphere reduced the hemolysis as well. These findings indicate an involvement of reactive oxygen species in the mechanism of action of the hemolytic process in the presence of oral antidiabetic drugs.
对磺酰胺衍生的口服抗糖尿病药物卡比马脲、氯磺丙脲、格列本脲、格列波脲、格列齐特、格列吡嗪、格列喹酮、格列索脲、格列嘧啶、妥拉磺脲和甲苯磺丁脲进行了体外光溶血特性研究。用SOL 3装置（太阳模拟照射）照射发现，氯磺丙脲、格列吡嗪、格列喹酮、格列嘧啶和甲苯磺丁脲（浓度均为10⁻³mol/l）存在时会发生溶血。除了格列嘧啶在浓度为10⁻⁴mol/l时会产生光溶血外，在10⁻⁴mol/l或10⁻⁵mol/l浓度下未观察到溶血作用。用TL 12灯泡（UVB）、UVASUN 5000装置（UVA）或实验灯（可见光）照射，任何一种受试物质均未诱导光毒性溶血。添加抗氧化剂抗坏血酸、α-生育酚或超氧化物歧化酶可显著抑制光毒性溶血。在富氮气氛中进行的研究也降低了溶血作用。这些发现表明，在口服抗糖尿病药物存在的情况下，活性氧参与了溶血过程的作用机制。
