Bonita R, Taira N
Veterinary Services Division, Office of the Provincial Agriculturist, Pasig City, Philippines.
Vet Parasitol. 1996 Dec 31;67(3-4):269-73. doi: 10.1016/s0304-4017(96)00990-9.
Faecal examination for Fasciola eggs which were preserved in 5% and 10% formalin and TAF solutions for 6 years was carried out using the Beads technique. The morphological characters and the distinctive yellowish color of Fasciola eggs were retained and appeared similar to those of fresh eggs. The mean egg recovery rate of eggs in TAF solution was significantly lower than the others (P < 0.01). The egg recovery rates from 5% and 10% formalin samples were 57.2% and 60.8%, respectively, and these values were similar to the value of 58.8% for fresh Fasciola eggs in cattle faeces. For Paramphistomum eggs, preserved with 5% formalin, the mean egg recovery rate was 64.7%. Fasciola eggs and Paramphistomum eggs were easily identified by their morphological characters in this experiment. The present data demonstrated that preservation with 5% formalin solution is an acceptable means of storing faecal samples for later examination of Fasciola and Paramphistomum eggs using the Beads technique and presumably by other fluke sedimentation methods.
采用珠式技术对保存在5%和10%福尔马林以及TAF溶液中6年的粪便样本进行肝片吸虫虫卵检查。肝片吸虫虫卵的形态特征和独特的淡黄色得以保留，与新鲜虫卵相似。TAF溶液中虫卵的平均回收率显著低于其他溶液（P < 0.01）。5%和10%福尔马林样本的虫卵回收率分别为57.2%和60.8%，这些数值与牛粪便中新鲜肝片吸虫虫卵58.8%的回收率相似。对于保存在5%福尔马林中的姜片吸虫虫卵，平均回收率为64.7%。在本实验中，通过形态特征可轻松鉴别肝片吸虫虫卵和姜片吸虫虫卵。目前的数据表明，用5%福尔马林溶液保存粪便样本是一种可接受的方法，可用于日后采用珠式技术以及大概其他吸虫沉淀法对肝片吸虫和姜片吸虫虫卵进行检查。
