孕妇弓形虫抗体滴度的酶联免疫吸附测定及间接乳胶凝集试验检测

Toxoplasma antibody titers by ELISA and indirect latex agglutination test in pregnant women.

作者信息

Ryu J S, Min D Y, Ahn M H, Choi H G, Rho S C, Shin Y J, Choi B, Joo H D

机构信息

Department of Parasitology, College of Medicine, Hanyang University, Seoul, Korea.

出版信息

Korean J Parasitol. 1996 Dec;34(4):233-8. doi: 10.3347/kjp.1996.34.4.233.

DOI:10.3347/kjp.1996.34.4.233

PMID:9017908

Abstract

The seroepidemiologic studies on anti-Toxoplasma antibody titers were carried out using ELISA and indirect latex agglutination test. Among 899 sera prepared from pregnant women, 39 cases (4.3%) revealed positive reaction and 218 sera from middle school students showed 4 positive reaction (1.8%) by ELISA. By LAT (newly established by National Veterinary Research Institute. Korea), the sera of 7 pregnant women (0.8%) showed positive reaction. When 80 sera showing > or = 1:8 by LAT were used for comparing the results obtained from LAT and Toxotest-MT (Eiken Chemical Co., Japan), 7 cases and 8 sera were positive, respectively. All of 11 sera of proven toxoplasmosis patients showed positive reaction in both tests. Overall proportion of agreement between LAT kit and Toxotest-MT was 0.94 (kappa-index = 0.632, p < 0.011, and LAT was considered to be useful for the screening of toxoplasmosis.

摘要

采用酶联免疫吸附测定（ELISA）和间接乳胶凝集试验开展了抗弓形虫抗体滴度的血清流行病学研究。在899份孕妇血清样本中，39例（4.3%）呈现阳性反应；218份中学生血清样本经ELISA检测显示4例阳性反应（1.8%）。通过乳胶凝集试验（由韩国国立兽医研究所新研发）检测，7名孕妇血清样本（0.8%）呈现阳性反应。当采用乳胶凝集试验中滴度≥1:8的80份血清样本对比乳胶凝集试验和弓形虫检测试剂盒MT（日本荣研化学株式会社）的检测结果时，分别有7例和8份血清样本呈阳性。11份确诊为弓形虫病患者的血清样本在两种检测中均呈阳性反应。乳胶凝集试验试剂盒和弓形虫检测试剂盒MT之间的总体一致性比例为0.94（kappa指数=0.632，p<0.011），且认为乳胶凝集试验对弓形虫病筛查有用。